STAT1 Antibody

Images

 
Western Blot: STAT1 Antibody [NBP1-77066] - Whole cell lysates from HeLa (H), Jurkat (J), A431 (A), K562 (K), and NIH3T3 (N) cells, with STAT1 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NBP1-77066] - HeLa cells with STAT1 antibody at 10 ug/ml.
Immunohistochemistry: STAT1 Antibody [NBP1-77066] - Analysis of STAT1alpha in human colon tissue with STAT1alpha antibody at 2.5 ug/ml.

Product Details

Summary
Product Discontinued
View other related STAT1 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-77066
    • Availability
      Product Discontinued

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STAT1 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The sequences differ from the murine corresponding sequences by four amino acids. The immunogen is located within the last 50 amino acids of STAT1 alpha.
Specificity
At least two isoforms of STAT1 are known to exist; this antibody will only recognize the larger isoform.
Clonality
Polyclonal
Host
Rabbit
Gene
STAT1
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
Application Notes
STAT1 alpha antibody can be used for Western blot at 1:1000 to 1:2000 and for immunoprecipitation at 2 to 4 ug per sample. A 91 kDa band can be detected. No reaction to the 84 kDa STAT1a.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for STAT1 Antibody

  • CANDF7
  • DKFZp686B04100
  • ISGF-3
  • ISGF-3,91kD
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription-1
  • STAT1
  • STAT91

Background

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-alpha, IFN-beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta which are encoded by the same gene with splicing variant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional STAT1 Products

Bioinformatics Tool for STAT1 Antibody (NBP1-77066)

Discover related pathways, diseases and genes to STAT1 Antibody (NBP1-77066). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for STAT1 Antibody (NBP1-77066)

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Research Areas for STAT1 Antibody (NBP1-77066)

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Blogs on STAT1.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

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Bioinformatics

Gene Symbol STAT1
Entrez
Uniprot