STAT1 Antibody (SAT-84)

Images

 
Western Blot: STAT1 Antibody (SAT-84) [NB100-78460] - Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse monoclonal antibody against STAT1. Proteins were visualized ...read more
Immunohistochemistry-Paraffin: STAT1 Antibody (SAT-84) [NB100-78460] - Formalin-fixed paraffin-embedded human tonsil tissue was stained with SAT-84 at 15 ug/ml and developed with an alkaline phosphatase chromogen ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78460
    • Availability
      Product Discontinued

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STAT1 Antibody (SAT-84) Summary

Immunogen
STAT1 peptide QLDSKFLEQVHQLYD
Specificity
STAT 1 (SAT-84)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
STAT1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 15 ug/ml
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2-4 ug/ml working concentration of antibody in 5% BSA for each mini-gel. For IHC:Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for STAT1 Antibody (SAT-84)

  • CANDF7
  • DKFZp686B04100
  • ISGF-3
  • ISGF-3,91kD
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription-1
  • STAT1
  • STAT91

Background

STAT1, also known as signal transduction and activator of transcription-1, is a ubiquitously expressed cytoplasmic protein that becomes phosphorylated in response to cytokine signaling (interferon alpha, interferon gamma, EGF, PDGF, and IL-6) by receptor-associated kinases and translocates to the nucleus to act as a transcriptional activator. Two isoforms of STAT1 exist as a result of alternative RNA processing with apparent molecular weights of 88 and 91 kD. STAT1 has been shown to interact with a number of proteins including STAT3, FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27 receptor, IL-2R beta and gamma, IFN alphaR beta, STAT2, c-Src, and many others. STAT1 knockout mice are highly sensitive to infection with microbial infections and viruses. The SAT-84 monoclonal antibody recognizes human STAT1 and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for STAT1 Antibody (NB100-78460)

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Blogs on STAT1.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

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Bioinformatics

Gene Symbol STAT1
Entrez
Uniprot