STAT1 Antibody (4H9)

Images

 
Western Blot: STAT1 Antibody (4H9) [NBP1-48011] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-STAT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.
Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human Kidney tissue using anti-STAT1 mouse monoclonal antibody.
Western Blot: STAT1 Antibody (4H9) [NBP1-48011] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Immunofluorescent staining of HT29 cells using anti-STAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human breast tissue using anti-STAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human endometrium tissue using anti-STAT1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
4H9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

STAT1 Antibody (4H9) Summary

Immunogen
Protein expressed in 293T cell transfected with human STAT1 expression vector (NP_009330).
Specificity
This antibody is specific for Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Reviewed Applications
Read 1 Review rated 4
using
NBP1-48011 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for STAT1 Antibody (4H9)

  • CANDF7
  • DKFZp686B04100
  • ISGF-3
  • ISGF-3,91kD
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription-1
  • STAT1
  • STAT91

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors,STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT1 Antibody (NBP1-48011) (0)

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Review for STAT1 Antibody (NBP1-48011) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-48011:
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WB
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Images Ratings Applications Species Date Details
Western Blot STAT1 NBP1-48011
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reviewed by:
rajesha rupaimoole
WB Human 10/06/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman ovarian cancer cell line A2780
SpeciesHuman

Blocking

Blocking Details5% BSA overnight

Primary Anitbody

Dilution Ratio1:1000

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STAT1 Antibody (NBP1-48011). (Showing 1 - 1 of 1 FAQ).

  1. We are looking for an NBS mouse monoclonal antibody that would work for IF. NBS1 Antibody (NB100-221) might be ideal, except we need to use it in mouse cells, and the data sheet says it is reactive with human cells. Could you tell us if it has been tested in mouse cells? If it has not, we would be willing to test it, and send you verifying images. However, if we test it and it does not work in mouse cells, would we be able to receive another antibody in exchange? We would also like to purchase a STAT1 mouse monoclonal antibody that would work for IF. STAT1 Antibody (NBP1-48011). The species reactivity of the antibody indicates that it is reactive with human, canine, and primate cells. Has it been tested in mouse cells?
    • I have a couple of thoughts after reading through your needs for this antibody. I assume you realize that if you want to use a mouse mAb on mouse tissue, you will need to do a mouse on mouse block to prevent non-specific staining with your secondary. If you decide that you would like to pursue this route, you do qualify for our Innovators Reward Program. Please see the link below. This might be a viable option for you if you want to test an antibody in an untested species. Another option would be for you to consider using a rabbit monoclonal instead, NB110-57272. This antibody has been verified for IF/ICC in mouse. The Innovator's Program would also apply to NBP1-48011.

Secondary Antibodies

 

Isotype Controls

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Research Areas for STAT1 Antibody (NBP1-48011)

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Blogs on STAT1.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

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Recent Reviews

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rajesha rupaimoole
10/06/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol STAT1
Entrez