ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Overexpression Lysate (Native)

Images

 
Western Blot: CD75 Overexpression Lysate (Adult Normal) [NBL1-16497] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD75.

Order Details


    • Catalog Number
      NBL1-16497
    • Availability
      Product Discontinued

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ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208061 and based on accession number NM_003032. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 2, mRNA.
Gene
ST6GAL1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Overexpression Lysate (Native)

  • Alpha 2,6-ST
  • B-cell antigen CD75
  • beta-galactoside alpha-2,6-sialyltransferase 1
  • CD75
  • CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase
  • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
  • EC 2.4.99.1
  • MGC48859
  • sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)
  • sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase)
  • Sialyltransferase 1
  • SIAT1
  • SIAT1Alpha 2,6-ST 1
  • ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
  • ST6Gal I
  • ST6GAL1
  • ST6GalI
  • ST6N

Background

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Lysate (NBL1-16497) (0)

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Bioinformatics

Gene Symbol ST6GAL1
Entrez