SSSCA1 Antibody (2F5)

Images

 
Western Blot: SSSCA1 Antibody (2F5) [NBP2-03198] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSSCA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: SSSCA1 Antibody (2F5) [NBP2-03198] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1.
Flow Cytometry: SSSCA1 Antibody (2F5) [NBP2-03198] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSSCA1 antibody, and then ...read more
Flow Cytometry: SSSCA1 Antibody (2F5) [NBP2-03198] - Analysis of Hela cells, using anti-SSSCA1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: SSSCA1 Antibody (2F5) [NBP2-03198] - Analysis of Jurkat cells, using anti-SSSCA1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
2F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SSSCA1 Antibody (2F5) Summary

Immunogen
Full length human recombinant protein of human SSSCA1 (NP_006387) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SSSCA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SSSCA1 Antibody (2F5)

  • Autoantigen p27
  • centromeric autoantigen (27kD)
  • p27
  • Sjoegren syndrome/scleroderma autoantigen 1
  • Sjogren syndrome/scleroderma autoantigen 1
  • Sjogren's syndrome/scleroderma autoantigen 1

Background

This antigen is recognized by a subset of anti-centromere antibodies from patients with scleroderma and/or Sjogren's syndrome. Subcellular localization has not yet been established. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SSSCA1
Entrez