SRY Antibody

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Western Blot: SRY Antibody [NB100-92365] - Western blot (WB) analysis of SRY (L80) antibody in extracts from HeLa cells.

Product Details

Summary
Product Discontinued
View other related SRY Primary Antibodies

Order Details


    • Catalog Number
      NB100-92365
    • Availability
      Product Discontinued

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SRY Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 50-100 of Human SRY.
Specificity
SRY (L80) detects endogenous levels of SRY (L80) protein.
Clonality
Polyclonal
Host
Rabbit
Gene
SRY
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of SRY (L80) pAb in extracts from HeLa cells.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SRY Antibody

  • essential protein for sex determination in human males
  • sex determining region Y
  • sex-determining region on Y
  • sex-determining region Y protein
  • TDFsex determining region protein
  • TDY
  • Testis-determining factor

Background

SRY (sex-determining region Y protein) is a transcriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SRY
Entrez