Splicing Factor 1 Antibody

Images

 
Western Blot: Splicing Factor 1 Antibody [NB100-92224] - Western blot (WB) analysis of Splicing Factor 1 (P76) antibody in extracts from HeLa cells treated with TNF-a 20ng/ml 2'.
Immunohistochemistry: Splicing Factor 1 Antibody [NB100-92224] - Immunohistochemistry (IHC) analyzes of Splicing Factor 1 (P76) Antibody in paraffin-embedded human brain tissue

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92224
    • Availability
      Product Discontinued

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Splicing Factor 1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 45-98 of Human SF1.
Specificity
Splicing Factor 1 (P76) detects endogenous levels of SF1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
SF1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Splicing Factor 1 (P76) pAb in extracts from HeLa cells treated with TNF-a 20ng/ml 2'.
Immunohistochemistry (IHC) analyzes of SF1 (P76) pAb in paraffin-embedded human brain tissue.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Splicing Factor 1 Antibody

  • Mammalian branch point-binding protein
  • mBBP
  • splicing factor 1
  • Transcription factor ZFM1
  • ZFM1BBP
  • Zinc finger gene in MEN1 locus
  • Zinc finger protein 162D11S636
  • ZNF162MBBP

Background

Steroidogenic factor-1 (SF-1) regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. Splicing Factor 1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. In the adult ovary, Splicing Factor 1 localizes to theca/interstitial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SF1
Entrez
Uniprot