SPG7 Antibody (1C1)

Images

 
Western Blot: SPG7 Antibody (1C1) [NBP2-01860] - Human tissue.
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (1C1) [NBP2-01860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Flow Cytometry: SPG7 Antibody (1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: SPG7 Antibody (1C1) [NBP2-01860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Western Blot: SPG7 Antibody (1C1) [NBP2-01860] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (1C1) [NBP2-01860] - Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.
Flow Cytometry: SPG7 Antibody (1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
1C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SPG7 Antibody (1C1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7(NP_003110) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPG7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 28131770).
Publications
Read Publication using
NBP2-01860 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SPG7 Antibody (1C1)

  • CARMGC126331
  • cell matrix adhesion regulator
  • CMARMGC126332
  • EC 3.4.24
  • EC 3.4.24.-
  • FLJ37308
  • paraplegin
  • PGNcell adhesion regulator
  • spastic paraplegia 7 (pure and complicated autosomal recessive)
  • Spastic paraplegia 7 protein
  • SPG5C

Background

This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPG7 Antibody (NBP2-01860)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPG7
Entrez