SP1 Antibody

Product Discontinued

SP1 Antibody Summary

• Immunogen: Synthetic peptide: CKDSEGRGSGDPGKKKQH corresponding to the interanl domain of human SP1.
• Localization: Nuclear
• Specificity: DB052 Sp1 (I18) reacts with Sp1of mouse, rat and human origin
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot 1/200
• Application Notes: In Western Blot predicted molecular weight 94 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.2% Gelatin, PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SP1 Antibody

• Sp1 transcription factor
• specificity protein 1
• transcription factor Sp1
• TSFP1

Background

The Sp (specificity protein) family is a family of transcription factors that bind to cis-elements in the promoter regions of various genes. Members of the family bind with varying affinities to sequences designated as 'Sp1 sites' (e.g., GC-boxes, CACCC-boxes, and basic transcription elements) (4,8). In human cells, telomerase activity is tightly regulated by the expression of human telomerase reverse transcriptase (hTERT). Sp1 and Sp3 have been shown to associate with the hTERT promoter, recruiting histone deacetylase for the localized deacetylation of nucleosomal histones and transcriptional silencing of the hTERT gene in normal human somatic cells (3). The role of the Sp family members in many diseases is being investigated. Sp-1 has been discussed in association with diabetic microvasculopathy and Huntington's disease (2,5). It has been suggested that transcription of the Sp3 gene is blocked in immune cells from most patients with multiple sclerosis and that this contributes to the development of central nervous system inflammation in the disease (6). Sp3 has also been shown to be required for proper skeletal ossification (7). Down-regulation of the transcription factor Sp-1 is thought to be involved in the inhibition of strain-induced mitogenesis in human vascular smooth muscle cells by estrogen via an estrogen receptor mediated process (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SP1
• Entrez:
  • 20683(Mouse)
  • 24790(Rat)