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SOX21 Antibody (2G10) [DyLight 405]

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Summary
Product Discontinued
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    • Catalog Number
      H00011166-M01V
    • Availability
      Product Discontinued

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SOX21 Antibody (2G10) [DyLight 405] Summary

Immunogen
SOX21 (NP_009015, 224 a.a. ~ 266 a.a) partial recombinant protein with GST tag.HSHPSPGNPGYMIPCNCSAWPSPGLQPPLAYILLPGMGKPQL
Specificity
SOX21 - SRY (sex determining region Y)-box 21
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SOX21
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
IgG purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SOX21 Antibody (2G10) [DyLight 405]

  • SOX21
  • SOX25
  • SOX25SOX-A
  • SOXA
  • SRY (sex determining region Y)-box 21
  • SRY-box 21
  • transcription factor SOX-21

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).  View our protocol for Staining Intracellular Molecules.
Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(11 Reviews)
(69 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
SOX3 Antibody
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SOX3 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Publication)
Species: Hu
Applications: WB, ICC
SOX1 Antibody
[Unconjugated]
AF3369
SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
SOX1 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(3 Reviews)
(19 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
SOX9 Antibody
[Unconjugated]
AF3075
SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO‑III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX9 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(4 Reviews)
(20 Publications)
Species: Hu
Applications: WB, ICC
SOX11 Antibody
NBP1-85823
Western Blot: SOX11 Antibody [NBP1-85823] - Analysis in human cell line SH-SY5Y.
Immunohistochemistry-Paraffin: SOX11 Antibody [NBP1-85823] - Immunohistochemical staining of human chronic lymphocytic leukemia shows no nuclear positivity in tumor cells as expected.
Immunohistochemistry-Paraffin: SOX11 Antibody [NBP1-85823] - Immunohistochemical staining of human mantle cell lymphoma shows moderate to strong nuclear positivity in tumor cells.
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SOX14
Overexpression Lysate
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Western Blot: SOX14 Overexpression Lysate (Adult Normal) [NBP2-07901] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SOX14.
Species: Hu
SRY Antibody (1G4)
NBP2-37463
Western Blot: SRY Antibody (1G4) [NBP2-37463] - Western blot analysis using SRY mouse mAb against NTERA-2 (1) cell lysate.
Immunohistochemistry: SRY Antibody (1G4) [NBP2-37463] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SRY mouse mAb with DAB staining.
Flow Cytometry: SRY Antibody (1G4) [NBP2-37463] - Flow cytometric analysis of HepG2 cells using SRY mouse mAb (green) and negative control (red).
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready
OCT4 Antibody
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Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.
Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB100-2379] - OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody.
Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat with OCT4 Antibody NB100-2379 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
(8 Publications)
Species: Hu, Mu, Rt, Pm
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SOX10 Antibody
[Unconjugated]
AF2864
SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(2 Reviews)
(13 Publications)
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BBS9 Antibody
NBP1-88927
Immunocytochemistry/Immunofluorescence: BBS9 Antibody [NBP1-88927] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm.
Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
(3 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SOX5 Antibody
AF5286
Western blot shows lysates of human lung tissue and exponentially growing LNCaP human prostate cancer cell line whole cell extract. PVDF membrane was probed with 1 µg/mL of Human SOX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5286) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
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SOX30 Antibody
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Western Blot: SOX30 Antibody [NBP1-86503] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503] - Staining in human testis and prostate tissues using anti-SOX30 antibody. Corresponding SOX30 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503] - Staining of human prostate shows low expression as expected.
Species: Hu
Applications: WB, IHC, IHC-P
SOX17 Antibody
[Unconjugated]
AF1924
Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(5 Reviews)
(83 Publications)
Species: Hu
Applications: WB, Simple Western, ChIP, ICC
SPANXB1 Antibody
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Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
YB1 Antibody
NBP1-97572
Western Blot: YB1 Antibody [NBP1-97572] - Western blot analysis of YB1 expression in 1) HeLa and 2) NTera2 whole cell lysates using NBP1-97572.
Immunocytochemistry/Immunofluorescence: YB1 Antibody [NBP1-97572] - The YB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin (NB100-690) were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: YB1 Antibody [NBP1-97572] - IHC staining of YB1 in mouse testis using DAB with hematoxylin counterstain.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
PLEKHM1 Antibody
NBP1-83013
Western Blot: PLEKHM1 Antibody [NBP1-83013] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP1-83013] - Staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Species: Hu
Applications: WB, IHC, IHC-P
SOX4 Antibody
(CL5634)
NBP2-61420
Immunocytochemistry/Immunofluorescence: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of rat hippocampus shows strong nuclear immunoreactivity in a subset of cells in the granule cell layer of the dentate gyrus.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
Fibronectin
Antibody
NBP1-91258
Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in NIH 3T3 cell lysate.
Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Fibronectin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-91258] - analysis of Fibronectin in human oesophageal cancer tissue using anti-Fibronectin antibody. Image from verified customer review.
(5 Reviews)
(9 Publications)
Species: Hu, Mu, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol SOX21
Entrez