SOX13 Antibody (2559A) [Unconjugated]

SOX13 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human SOX13 Monoclonal Antibody (Catalog # MAB5118) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei and cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 2559A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SOX13 Antibody (2559A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human SOX13
  Met19-Pro258
  Accession # Q9UN79
• Specificity: Detects human SOX13 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX13 Antibody (2559A) [Unconjugated]

• ICA12
• Islet cell antigen 12
• MGC117216
• SOX13
• Sox-13
• SRY (sex determining region Y)-box 13ICA12islet cell 12
• SRY-box 13
• SRY-related HMG-box gene 13
• transcription factor SOX-13
• Type 1 diabetes autoantigen ICA12

Background

SOX-13 is a transcription factor involved in the embryonic development and the determination of cell fate.  It is also known as islet cell antibody 12 since it has been determined to be a type-1 diabetes autoantigen.  SOX-13 RNA is detected in most human tissues with highest levels in the pancreas, placenta, and kidney.  Immunohistochemistry on human pancreas identifies SOX-13 in the islets of Langerhans where staining is mostly cytoplasmic but can also be nuclear. SOX-13 antibody testing helps identify latent autoimmune diabetes of adults (LADA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9UN79(Human)