SOX10 Antibody (SOX10/991) - Azide and BSA Free

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Western Blot: SOX10 Antibody (SOX10/991) - Azide and BSA Free [NBP2-59621] - analysis of SOX10 (A) Recombinant protein (B) A375 Cell lysate using SOX10 Mouse Monoclonal Antibody (SOX10/991).
Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/991) - Azide and BSA Free [NBP2-59621] - Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX10 Mouse Monoclonal Antibody (SOX10/991).
Flow (Intracellular): SOX10 Antibody (SOX10/991) - Azide and BSA Free [NBP2-59621] - An intracellular stain was performed on SK-MEL-28 cells with SOX10 Antibody [SOX10/991] NBP2-59621PE (blue) and a matched isotype ...read more
Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/991) - Azide and BSA Free [NBP2-59621] - Formalin-fixed, paraffin-embedded Human Melanoma stained with SOX10 Mouse Monoclonal Antibody (SOX10/991).
Flow (Intracellular): SOX10 Antibody (SOX10/991) - Azide and BSA Free [NBP2-59621] - An intracellular stain was performed on SK-MEL-28 cells with SOX10 [SOX10/991] Antibody NBP2-59621AF488 (blue) and a matched isotype ...read more
Protein Array: SOX10 Antibody (SOX10/991) - Azide and BSA Free [NBP2-59621] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, IHC, IHC-P, PA, CyTOF-ready
Clone
SOX10/991
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

SOX10 Antibody (SOX10/991) - Azide and BSA Free Summary

Description
Human Chromosome Location:22q13.1
Positive Control: HepG2 cells. Melanomas, breast carcinomas, gliomas
Immunogen
Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)
Localization
Nuclear
Marker
Melanoma Marker
Specificity
The specificity of this monoclonal antibody to human, mouse SOX10 was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG -box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SOX10
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1 ug/ml
  • Protein Array
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for SOX10 Antibody (SOX10/991) - Azide and BSA Free

  • DOM
  • MGC15649
  • PCWH
  • SOX10
  • SRY (sex determining region Y)-box 10
  • SRY-related HMG-box gene 10
  • transcription factor SOX-10
  • WS4
  • WS4C
  • WS4mouse, human homolog of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SOX10 Antibody (NBP2-59621) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-59621AF350
Alexa Fluor 405 NBP2-59621AF405
Alexa Fluor 488 NBP2-59621AF488
Alexa Fluor 532 NBP2-59621AF532
Alexa Fluor 594 NBP2-59621AF594
Alexa Fluor 647 NBP2-59621AF647
Alexa Fluor 700 NBP2-59621AF700
Alexa Fluor 750 NBP2-59621AF750
Allophycocyanin NBP2-59621APC
Biotin NBP2-59621B
DyLight 350 NBP2-59621UV
DyLight 405 NBP2-59621V
DyLight 488 NBP2-59621G
DyLight 550 NBP2-59621R
DyLight 594 NBP2-59621DL594
DyLight 650 NBP2-59621C
DyLight 680 NBP2-59621FR
DyLight 755 NBP2-59621IR
FITC NBP2-59621F
HRP NBP2-59621H
Janelia Fluor 549 NBP2-59621JF549
Janelia Fluor 646 NBP2-59621JF646
PE NBP2-59621PE
PerCP NBP2-59621PCP

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Bioinformatics

Gene Symbol SOX10