Sonic Hedgehog/Shh Antibody (RM0128-4A37)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-22505
    • Availability
      Product Discontinued

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Sonic Hedgehog/Shh Antibody (RM0128-4A37) Summary

Immunogen
Mouse recombinant protein of Sonic Hedgehog (Shh) C-Terminal fragment
Specificity
This was produced from a hybridoma (mouse myeloma) fused with spleen cells from a rat immunized with mouse recombinant protein of the Sonic Hedgehog (Shh) C-Terminal fragment.
Isotype
IgG2
Clonality
Monoclonal
Host
Rat
Gene
SHH
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
This antibody detects mouse Shh, but not dhh or ihh in Western blotting.

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.2 ml sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for Sonic Hedgehog/Shh Antibody (RM0128-4A37)

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

Sonic Hedgehog, which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol SHH
Entrez