Sonic Hedgehog/Shh Antibody (8G3)

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Western Blot: Sonic Hedgehog/Shh Antibody (8G3) [NBP1-51615] - Western blot analysis using SHH mAb against SHH-hIgGFc transfected HEK293 cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
8G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Sonic Hedgehog/Shh Antibody (8G3) Summary

Immunogen
Purified recombinant fragment of human SHH expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SHH
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:1000
  • ELISA 1:10000
Theoretical MW
49.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Sonic Hedgehog/Shh Antibody (8G3)

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

SHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SHH
Entrez