Sonic Hedgehog/Shh Antibody (3A2)

Images

 
Western Blot: Sonic Hedgehog Antibody (3A2) [NBP1-47965] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Sonic Hedgehog(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog Antibody (3A2) [NBP1-47965] Staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY Sonic Hedgehog.

Product Details

Summary
Product Discontinued
View other related Sonic Hedgehog/Shh Primary Antibodies

Order Details


    • Catalog Number
      NBP1-47965
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Sonic Hedgehog/Shh Antibody (3A2) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 24-197 of human SHH
Specificity
This antibody is specific for Homo sapiens sonic hedgehog homolog (Drosophila) (SHH).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SHH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-47965 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Sonic Hedgehog/Shh Antibody (3A2)

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

SHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-600
Species: Hu, Mu
Applications: WB, ChIP, ELISA, IHC, IHC-P, Flow-IC
NBP1-47945
Species: Hu, Mk
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-57016
Species: Hu
Applications: WB
H00003077-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
423-F8/CF
Species: Hu, Mu
NBP2-24543
Species: Hu, Mu, Pm
Applications: WB, IHC-P
NBP2-55775
Species: Hu
Applications: WB, ICC/IF
NBP2-24543
Species: Hu, Mu, Pm
Applications: WB, IHC-P
AF3690
Species: Hu, Mu
Applications: WB, ChIP, ICC
NBP2-23602
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
1705-HH/CF
Species: Hu, Mu
355-BM/CF
Species: Hu, Mu, Rt
NBP1-33581
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
AF482
Species: Mu
Applications: WB, IHC
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-33404
Species: Hu
Applications: IHC, IHC-P
NBP1-47965
Species: Hu
Applications: WB, ICC/IF

Publications for Sonic Hedgehog/Shh Antibody (NBP1-47965)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Sonic Hedgehog/Shh Antibody (NBP1-47965) (0)

There are no reviews for Sonic Hedgehog/Shh Antibody (NBP1-47965). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sonic Hedgehog/Shh Antibody (NBP1-47965) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Sonic Hedgehog/Shh Products

Bioinformatics Tool for Sonic Hedgehog/Shh Antibody (NBP1-47965)

Discover related pathways, diseases and genes to Sonic Hedgehog/Shh Antibody (NBP1-47965). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Sonic Hedgehog/Shh Antibody (NBP1-47965)

Discover more about diseases related to Sonic Hedgehog/Shh Antibody (NBP1-47965).
 

Pathways for Sonic Hedgehog/Shh Antibody (NBP1-47965)

View related products by pathway.

PTMs for Sonic Hedgehog/Shh Antibody (NBP1-47965)

Learn more about PTMs related to Sonic Hedgehog/Shh Antibody (NBP1-47965).
 

Research Areas for Sonic Hedgehog/Shh Antibody (NBP1-47965)

Find related products by research area.

Blogs on Sonic Hedgehog/Shh

There are no specific blogs for Sonic Hedgehog/Shh, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Sonic Hedgehog/Shh Antibody (3A2) and receive a gift card or discount.

Bioinformatics

Gene Symbol SHH
Entrez
Uniprot