Sonic Hedgehog/Shh Antibody (10H6)

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Western Blot: Sonic Hedgehog Antibody (10H6) [NBP1-47966] Analysis of extracts (35ug) from 9 different cell lines by using anti-Sonic Hedgehog monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog Antibody (10H6) [NBP1-47966] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Sonic Hedgehog.
Western Blot: Sonic Hedgehog Antibody (10H6) [NBP1-47966] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Sonic Hedgehog (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF
Clone
10H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Sonic Hedgehog/Shh Antibody (10H6) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 24-197 of human SHH
Localization
Sonic hedgehog protein C-product: Secreted. The C-terminal peptide diffuses from the cell. Sonic hedgehog protein N-product: Cell membrane. The N-product either remains associated with lipid rafts at the cell surface or forms freely diffusable active multimers with its hydrophobic lipid-modified N- and C-termini buried inside.
Specificity
This antibody is specific for Homo sapiens sonic hedgehog homolog (Drosophila) (SHH).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SHH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Immunocytochemistry/Immunofluorescence 1:100
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Dog, Rat, Monkey.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Sonic Hedgehog/Shh Antibody (10H6)

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

SHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SHH
Entrez