Sodium Potassium ATPase Alpha 1 Antibody (M8-P1-A3)

Images

 
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (M8-P1-A3) [NB600-1458] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Frozen: Sodium Potassium ATPase Alpha 1 Antibody (M8-P1-A3) [NB600-1458] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (M8-P1-A3) [NB600-1458] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.

Order Details


    • Catalog Number
      NB600-1458
    • Availability
      Product Discontinued

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Sodium Potassium ATPase Alpha 1 Antibody (M8-P1-A3) Summary

Immunogen
Lamb kidney alpha 1 sodium/potassium ATPase.
Epitope
This antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.
Marker
Plasma Membrane Marker
Specificity
Detects sodium/potassium ATPase alpha 1. This does not detect the alpha 2 or alpha 3 isoform in rat.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ATP1A1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200 - 1:2000
  • Flow Cytometry 1:20 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100 - 1:2000
Application Notes
WB: Detects an approx. 110 kDa protein representing alpha 1 sodium/potassium ATPase from canine kidney extract. IHC: Staining of sodium / potassium ATPase alpha 1 yields a pattern consistent with plasma membrane localization.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites with PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Sodium Potassium ATPase Alpha 1 Antibody (M8-P1-A3)

  • ATPase, Na+/K+ transporting, alpha 1 polypeptide
  • EC 3.6.3
  • EC 3.6.3.9
  • K-ATPase alpha-1 subunit
  • K-ATPase catalytic subunit alpha-A protein
  • MGC3285
  • MGC51750
  • Na
  • Na(+)/K(+) ATPase alpha-1 subunit
  • Na, K-ATPase, alpha-A catalytic polypeptide
  • Na+, K+ ATPase alpha subunit
  • Na+/K+ ATPase 1
  • sodium pump 1
  • Sodium pump subunit alpha-1
  • sodium/potassium-transporting ATPase subunit alpha-1
  • sodium-potassium-ATPase, alpha 1 polypeptide

Background

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium / potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium / potassium ATPase exhibit tissue specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sodium Potassium ATPase Alpha 1 Antibody (NB600-1458). (Showing 1 - 1 of 1 FAQ).

  1. I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.
    • Our sodium potassium ATPase and plasma membrane products can be found using this links. I hope this helps. Please contact us at technical@novusbio.com with any additional questions.

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Bioinformatics

Gene Symbol ATP1A1
Entrez