Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified

Images

 
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified [NBP2-61137] - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified [NBP2-61137] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X ...read more
Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified [NBP2-61137] - Staining of human enodmetrial glands within the uterus using NB300-146. Note the absence of staining in the surrounding ...read more
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified [NBP2-61137] - An intracellular stain was performed on A549 cells with NBP2-61137AF647 (Blue) and a matched isotype control (Orange). Cells ...read more
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified [NBP2-61137] - Detection of ATPA1 (Green) in HepG2 cells using NB300-146. Nuclei (Blue) were counterstained using ...read more
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified [NBP2-61137] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X ...read more
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified [NBP2-61137] - An intracellular stain was performed on A549 cells with - Purified NBP2-61137 and a matched isotype control. Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Dr, GP, Pm, Rb, Xp, YeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
464.6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Purified
Concentration
1 mg/ml

Order Details

Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified Summary

Immunogen
Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla. [UniProt# Q9N0Z6]
Marker
Plasma Membrane Marker
Specificity
This is specific for Na,K-ATPase alpha 1 subunit.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ATP1A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes
In Western Blot, a distinct band at ~ 112 kDa is seen. Do not boil the sample prior to loading on the gel for Western Blot (60 degrees Celsius appears to work fine). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26941236). Publication with sheep shown (PMID: 18424241); however results may vary. Use in immunoprecipitation reported in scientific literature (PMID: 27748972).
Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Ret Antibody (4G1)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Purified

  • ATPase, Na+/K+ transporting, alpha 1 polypeptide
  • EC 3.6.3
  • EC 3.6.3.9
  • K-ATPase alpha-1 subunit
  • K-ATPase catalytic subunit alpha-A protein
  • MGC3285
  • MGC51750
  • Na
  • Na(+)/K(+) ATPase alpha-1 subunit
  • Na, K-ATPase, alpha-A catalytic polypeptide
  • Na+, K+ ATPase alpha subunit
  • Na+/K+ ATPase 1
  • sodium pump 1
  • Sodium pump subunit alpha-1
  • sodium/potassium-transporting ATPase subunit alpha-1
  • sodium-potassium-ATPase, alpha 1 polypeptide

Background

Sodium Potassium ATPase alpha 1 (ATP1A1) belongs to the P-type ATPase family and is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with exchange of sodium (Na+) and potassium (K+ ) ions across the cellular plasma membranes. This action creates an electrochemical gradient of Na+ and K+ ions, providing the energy for active transport of various nutrients. Its phosphorylation at Tyr-10 position alters the pumping activity whereas dephosphorylation mediated by PP2A (protein phosphatase 2A) following increases in intracellular Na+, leads to catalytic activity. Because the plasma membrane is highly permeable to water, it is the concentration of ions across this membrane that is critical for maintaining an adequate cell volume and sodium potassium ATPase is key player in this maintainance process because it provides the driving force for active Na+ as well as K+ transport into/out of the cell, with water following isosmotically. This pump is consists of equimolar ratios of two main subunits, the catalytic alpha subunit (which contains the binding sites for the mentioned cations, ATP, and cardiotonic steroid inhibitors) and the regulatory beta polypeptides (which is essential for the normal activity of the enzyme as well as involved potentially in the occlusion of K+ and modulation of the enzyme's K+ /Na+ affinity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Sodium Potassium ATPase Alpha 1 Antibody (NBP2-61137) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sodium Potassium ATPase Alpha 1 Antibody (NBP2-61137). (Showing 1 - 1 of 1 FAQ).

  1. I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.
    • Our sodium potassium ATPase and plasma membrane products can be found using this links. I hope this helps. Please contact us at technical@novusbio.com with any additional questions.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-61137AF350
Alexa Fluor 405 NBP2-61137AF405
Alexa Fluor 488 NBP2-61137AF488
Alexa Fluor 532 NBP2-61137AF532
Alexa Fluor 594 NBP2-61137AF594
Alexa Fluor 647 NBP2-61137AF647
Alexa Fluor 700 NBP2-61137AF700
Alexa Fluor 750 NBP2-61137AF750
Allophycocyanin NBP2-61137APC
Biotin NBP2-61137B
DyLight 350 NBP2-61137UV
DyLight 405 NBP2-61137V
DyLight 488 NBP2-61137G
DyLight 550 NBP2-61137R
DyLight 650 NBP2-61137C
DyLight 680 NBP2-61137FR
DyLight 755 NBP2-61137IR
FITC NBP2-61137F
HRP NBP2-61137H
Janelia Fluor 549 NBP2-61137JF549
Janelia Fluor 646 NBP2-61137JF646
PE NBP2-61137PE
PerCP NBP2-61137PCP

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Bioinformatics

Gene Symbol ATP1A1