SOD3/EC-SOD Antibody (4G11G6)

Images

 
Western Blot: SOD3/EC-SOD Antibody (4G11G6) [NBP1-22417] - analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6. Primary Antibody: Mouse ...read more
Immunohistochemistry: SOD3/EC-SOD Antibody (4G11G6) [NBP1-22417] - analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6. Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
4G11G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SOD3/EC-SOD Antibody (4G11G6) Summary

Immunogen
Human extracellular SOD purified from aortas.
Localization
Extracellular Space
Specificity
Detects extracellular SOD approx 35kDa.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SOD3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
Application Notes
1 ug/ml of SOD (EC) Antibody was sufficient for detection of EC-SOD in 20 ug of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Publications
Read Publications using
NBP1-22417 in the following applications:

  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Based on homology, It's predicted to detect Mouse, Rat and Guinea Pig.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SOD3/EC-SOD Antibody (4G11G6)

  • ECSOD
  • EC-SOD
  • EC-SODEC 1.15.1.1
  • extracellular superoxide dismutase [Cu-Zn]
  • MGC20077
  • SOD3
  • superoxide dismutase 3, extracellular

Background

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOD3/EC-SOD Antibody (NBP1-22417)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOD3/EC-SOD Antibody (NBP1-22417). (Showing 1 - 1 of 1 FAQs).

  1. I've been using your mouse monoclonal anti-SOD3 antibody for a number of applications and have been very pleased with the results. However, I want to know what would be a good positive control for WB. The datasheet says that human aorta was used as the immunogen. However, I read that lung and kidney lysates make good positive controls for other anti-SOD3 antibodies. Would lung/kidney tissue work as a positive control for this particular antibody?
    • SOD3 protein is mainly expressed in blood vessels, heart, lung, kidney and placenta, and it is the major SOD isoenzyme found in extracellular fluids such as plasma, lymph and synovial fluid. So using lung or kidney as a positive control lysate would be appropriate for your immunoassays including Western blot.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-22417AF405
Alexa Fluor 488 Labeled NBP1-22417AF488
Alexa Fluor 647 Labeled NBP1-22417AF647
Alexa Fluor 700 Labeled NBP1-22417AF700
DyLight 350 Labeled NBP1-22417UV
DyLight 405 Labeled NBP1-22417V
DyLight 488 Labeled NBP1-22417G
DyLight 680 Labeled NBP1-22417FR
DyLight 755 Labeled NBP1-22417IR

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Bioinformatics

Gene Symbol SOD3
Entrez
Uniprot