Western Blot: SOD3/EC-SOD Antibody (4G11G6) [NBP1-22417] - analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6. Primary Antibody: Mouse ...read more
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for SOD3/EC-SOD Antibody (4G11G6)
extracellular superoxide dismutase [Cu-Zn]
superoxide dismutase 3, extracellular
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for SOD3/EC-SOD Antibody (NBP1-22417). (Showing 1 - 1 of 1 FAQs).
I've been using your mouse monoclonal anti-SOD3 antibody for a number of applications and have been very pleased with the results. However, I want to know what would be a good positive control for WB. The datasheet says that human aorta was used as the immunogen. However, I read that lung and kidney lysates make good positive controls for other anti-SOD3 antibodies. Would lung/kidney tissue work as a positive control for this particular antibody?
SOD3 protein is mainly expressed in blood vessels, heart, lung, kidney and placenta, and it is the major SOD isoenzyme found in extracellular fluids such as plasma, lymph and synovial fluid. So using lung or kidney as a positive control lysate would be appropriate for your immunoassays including Western blot.