SOCS-5 Antibody

Images

 
Immunohistochemistry: SOCS5 Antibody [NB600-1166] - IHC: Localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain in cardiac myocytes within the heart.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

SOCS-5 Antibody Summary

Immunogen
Synthetic peptide corresponding to C-terminus ofhuman / mouse SOCS 5.
Specificity
Thisreacts predominantly with SOCS 5. The antibodies were evaluated for specificity with a dot blot assay usingsynthetically prepared SOCS peptides. It onlyrecognizes SOCS 5, not other SOCS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOCS5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Immunoprecipitation: use at a concentration of 3.0 - 5.0 ug / extract from 10<sup>7</sup> cells.Western Blotting: use at a concentration of 0.5 - 2.0 ug/ml.ELISA: use at a concentration of 0.1 - 1.0 ug/ml. By Western blot, this antibody detects a band of approximately 64 kDa, which corresponds to the predicted molecular weight of SOCS 5.

Reactivity Notes

Cross-reacts with Human and Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOCS-5 Antibody

  • CIS6
  • CIS6Cytokine-inducible SH2 protein 6
  • CISH5
  • CISH6
  • CISH6CIS-6
  • KIAA0671Cytokine-inducible SH2-containing protein 5
  • SOCS5
  • SOCS-5
  • SOCS-5CISH5
  • suppressor of cytokine signaling 5

Background

The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. During Th1 differentiation a reduction in the association of Jak1 with the IL4 Receptor correlated with the appearance of SOCS5. SOCS5 protein was preferentially expressed in committed Th1 cells and interacted with the cytoplasmic region of the IL4 Receptor alpha chain irrespective of receptor tyrosine phosphorylation. This unconventional interaction of SOCS5 protein with IL4 Receptor resulted in the inhibition of IL4-mediated signal transducer and activator of transcription-6 activation. T cells from transgenic mice constitutively expressing SOCS5 exhibited a significant reduction of IL4-mediated Th2 development. Therefore, the induced SOCS5 protein in Th1 differentiation environment may play an important role by regulating Th1 and Th2 balance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SOCS5
Entrez