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Snx6 Antibody

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Western Blot: Snx6 Antibody [NBP2-94535] - Analysis of extracts of various cell lines, using Snx6 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.
Western Blot: Snx6 Antibody [NBP2-94535] - Analysis of extracts of various cell lines, using Snx6 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Snx6 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human SNX6 (NP_067072.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SNX6
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Snx6 Antibody

  • MGC3157
  • MSTP010
  • sorting nexin 6
  • sorting nexin-6
  • TFAF2
  • TRAF4-associated factor 2
  • tumor necrosis factor receptor-associated factor 4(TRAF4)-associated factor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SNX1 Antibody
(JG40-06)
NBP2-75670
Flow Cytometry: SNX1 Antibody (JG40-06) [NBP2-75670] - Analysis of SiHa cells with SNX1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: SNX1 Antibody (JG40-06) [NBP2-75670] - Staining SNX1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: SNX1 Antibody (JG40-06) [NBP2-75670] - Staining SNX1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TRAF-4 Antibody
NB100-56177
Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177] - Formalin-fixed, paraffin embedded, normal human mammary epithelial tissue using th antibody. A-C: Strong TRAF4 staining is seen in the luminal epithelial cells of the interlobular terminal ducts (acini). However, there is absence of TRAF4 immunostaining in the myoepithelial cells along the basement membrane. B and C: higher magnifications of A. Data courtesy of Krajewska et al.
Immunoprecipitation: TRAF-4 Antibody [NB100-56177] - The NB100-56177 TRAF4 antibody specifically immunoprecipitates TRAF4. Top: TRAFs 2,3,4,5, and 6 were in vitro translated in the presence of 35S-methionine, and immunoprecipiated using the TRAF4 antibody. The TRAF4 antibody immunoprecipitated only TRAF4 and did not recognize TRAFs 2, 3, 5, or 6. A reticulocyte primed with an empty pcDNA plasmid was used as a negative control. Bottom: SDS-PAGE visualization of the in vitro translated TRAFs. Data courtesy of Krajewska et al.
(1 Publication)
Species: Bv, Hu, Mu, Rt, Ze
Applications: IHC, IHC-P, IP, WB
Annexin A7 Antibody
AF3926
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Annexin A7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3926) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Species: Hu, Mu, Rt
Applications: WB
SNX5 Antibody
NB100-1362
Western Blot: SNX5 Antibody [NB100-1362] - Staining (1ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SNX5 Antibody [NB100-1362] - Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
IGF-II R/IGF2R
Antibody (2G11) - BSA ...
NB300-514
Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - An intracellular stain was performed on MCF7 cells with IGF-II R/IGF2R [2G11] Antibody NB300-514AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-IGF-II R/IGF2R Antibody (2G11) NB300-514 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mannose 6-Phosphate Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
(9 Publications)
Species: Bv, Hu, Mu, Pm, Rt
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
BRMS1 Antibody
(2D4-2G11)
H00025855-M01
Immunoprecipitation: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - Analysis of BRMS1 transfected lysate using anti-BRMS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BRMS1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - Detection limit for recombinant GST tagged BRMS1 is approximately 3ng/ml as a capture antibody.
Western Blot: BRMS1 Antibody (2D4-2G11) [H00025855-M01] - Analysis of BRMS1 expression in transfected 293T cell line by BRMS1 monoclonal antibody (M01), clone 2D4-2G11.Lane 1: BRMS1 transfected lysate (Predicted MW: 28.5 KDa).Lane 2: Non-transfected lysate.
(10 Publications)
Species: Hu
Applications: ELISA, IHC, IHC-P, IP, S-ELISA, WB
VPS26A Antibody -
BSA Free
NBP2-75922
Western Blot: VPS26 Antibody [NBP2-75922] - Total protein from A431, HeLa, and HepG2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-VPS26A in 2.5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: VPS26 Antibody [NBP2-75922] - IHC analysis of a formalin fixed paraffin embedded tissue section of the mouse testis using 1:350 dilution of VPS26A antibody (NBP2-75922). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.
Immunocytochemistry/Immunofluorescence: VPS26A Antibody [NBP2-75922] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-VPS26A Antibody NBP2-75922 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
VPS35 Antibody
NB100-1397
Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Confocal microscopy image of human RPE-1 cells fixed with PFA, permeablilized with 0.3% Triton X-100,then immunostained with vps35 antibody at 1:2000 overnight in 1% BSA at 4 degrees. ICC image submitted by a verified customer review.
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
(5 Reviews)
(23 Publications)
Species: Ca, Ch, ChHa, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
EGFR Antibody
[Unconjugated]
AF231
EGFR was detected in immersion fixed frozen sections of human skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
EGFR was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(13 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
SART3 Antibody
NB100-74648
Immunohistochemistry-Paraffin: SART3 Antibody [NB100-74648] - FFPE section of human pancreatic islet cell tumor. Affinity purified rabbit anti-SART3 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: SART3 Antibody [NB100-74648] - FFPE section of human pancreatic islet cell tumor. Affinity purified rabbit anti-SART3 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
Western Blot: SART3 Antibody [NB100-74648] - Detection of Human SART3/TIP110 on HeLa whole cell lysate using NB100-74648. SART3 was also immunoprecipitated by rabbit anti-SART3 antibody NB100-74649.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
beta Amyloid
Antibody (MOAB-2) - B ...
NBP2-13075
Immunohistochemistry-Frozen: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Mouse brain (cerebral cortex). Red: MOAB-2 antibody staining, Blue: DAPI. Zeiss LSM800, 40x. Image from verified customer review.
Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - A plaque load in neocortex and hippocampus in APP/PS1 mice exposed to differential housing. Representative 10x MOAB-2 staining in hippocampus of APP/PS1 mice for each housing group. Environmental novelty exacerbates stress hormones and Aβ pathology in an Alzheimer's model. Sci Rep (2017)
Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).
(2 Reviews)
(48 Publications)
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(36 Reviews)
(719 Publications)
Species: Hu
Applications: BA
OAS3 Antibody
NBP1-85841
Immunocytochemistry/Immunofluorescence: OAS3 Antibody [NBP1-85841] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: OAS3 Antibody [NBP1-85841] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemistry-Paraffin: OAS3 Antibody [NBP1-85841] - Staining of human duodenum shows weak cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
RP9 Antibody
NBP1-87342
Immunohistochemistry-Paraffin: RP9 Antibody [NBP1-87342] - Staining of human hippocampus shows nuclear positivity in glial cells and neurons.
Immunocytochemistry/Immunofluorescence: RP9 Antibody [NBP1-87342] - Staining of human cell line U-2 OS shows localization to nucleus & cytosol.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
TPX2 Antibody
NB500-179
Flow Cytometry: TPX2 Antibody [NB500-179] - RHAMM is not a cell surface but an intracellular cytoskeletal protein in mouse embryonic stem (ES) cells. (C) Alcohol permeabilized mouse ES cells, however, were strongly positive for both RHAMM and the intracellular positive control protein TPX2 (right panel). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0073548) licensed under a CC-BY licence.
Simple Western: TPX2 Antibody [NB500-179] - Lane view shows a specific band for TPX2 using HeLa cell lysate and antibody at 1:250. Electropherogram image of corresponding Simple Western lane view. Image reported by internal validation.
Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-179] - Staining of HeLa cells fixed in 3.5% paraformaldehyde using NB 500-179 (1:1,000). Nuclear staining during interphase and spindle staining during mitosis.
(1 Review)
(41 Publications)
Species: Hu, Ma, Mu, Po, Rt
Applications: Flow, ICC/IF, IP, KD, Simple Western, WB
LONP1 Antibody
NBP1-81734
Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human duodenum shows granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human fallopian tube shows granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human pancreas shows granular cytoplasmic positivity.
(13 Publications)
Species: Dr, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, Simple Western, WB
CDP/CUTL1 Antibody
(2A10)
H00001523-M01
Western Blot: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Analysis of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody (M01), clone 2A10.Lane 1: CUX1 transfected lysate(77.2 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - mouse cortex - postnatal brain was stained with anti-CUTL1 antibody. Image from verified customer review.
ELISA: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Detection limit for recombinant GST tagged CUTL1 is approximately 0.03ng/ml as a capture antibody.
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
ASCC2 Antibody
(OTI1E8) - Azide and ...
NBP2-72273
Western Blot: ASCC2 Antibody (OTI1E8) - Azide and BSA Free [NBP2-72273] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC2.
Flow Cytometry: ASCC2 Antibody (OTI1E8) - Azide and BSA Free [NBP2-72273] - Analysis of Hela cells, using anti-ASCC2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunocytochemistry/Immunofluorescence: ASCC2 Antibody (OTI1E8) - Azide and BSA Free [NBP2-72273] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ASCC2.
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, WB
PIM1 Antibody
NBP2-31366
Immunohistochemistry-Paraffin: PIM1 Antibody [NBP2-31366] - IHC-P analysis of PIM1 protein in a section of human breast normal tissue using PIM1 antibody at a concentration of 5 ug/ml. The breast ductal or acinar epithelial cells showed strong cytoplasmic as well as nuclear expression, whereas the myoepithelial cells and the intra-lobular connective tissue depicted weak PIM1 positivity.
Immunohistochemistry-Paraffin: PIM1 Antibody [NBP2-31366] - IHC-P analysis of PIM1 protein in a section of human esophageal squamous cell carcinoma (SCC) using PIM1 antibody at a concentration of 5 ug/ml. The representative image shows a strong cytoplasmic/nuclear staining of the SCC cells with moderate positivity in the tumor stroma.
Western Blot: PIM1 Antibody [NBP2-31366] - Western blot analysis of HeLa (A) and A431 (B) cell lysate using PIM1 antibody at a concentration of 2 ug/ml.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Snx6 Antibody
NBP2-94535
Snx6 Antibody
Species: Hu, Mu, Rt
Applications: WB

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Bioinformatics

Gene Symbol SNX6