Snail Antibody (5E12)

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Western Blot: SNAIL Antibody (5E12) [NBP1-47992] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAIL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more

Product Details

Summary
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Order Details


    • Catalog Number
      NBP1-47992
    • Availability
      Product Discontinued

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Snail Antibody (5E12) Summary

Immunogen
Protein expressed in 293T cell transfected with human SNAI1 expression vector
Specificity
This antibody is specific for Homo sapiens snail homolog 1 (Drosophila) (SNAI1).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SNAI1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Snail Antibody (5E12)

  • Protein sna
  • Protein snail homolog 1
  • SLUGH2
  • SNA
  • SNAH
  • SNAHdJ710H13.1
  • SNAI1
  • snail 1 (drosophila homolog), zinc finger protein
  • snail 1 homolog
  • snail 1 zinc finger protein
  • snail 1, zinc finger protein
  • snail homolog 1 (Drosophila)
  • Snail
  • zinc finger protein SNAI1

Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Snail Antibody (NBP1-47992). (Showing 1 - 1 of 1 FAQ).

  1. There are many kinds of antibodies you supply against SNAIL. Could you give me your recommendation? Which is the best for the IHC-P experiments the species of my samples to be tested is human.
    • Our SNAIL antibody with catalog # NBP1-19529 has been successfully validated for IHC-P in paraffin-embedded human lung carcinoma tissue and I would highly recommend you to use the same for your samples too. The working dilutions of this antibody for IHC-P ranges from 1:50 - 1:200 and beside IHC, you can use this antibody for Western Blot and Immunofluorescence also.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Snail Antibody (NBP1-47992)

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Research Areas for Snail Antibody (NBP1-47992)

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Blogs on Snail.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

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Bioinformatics

Gene Symbol SNAI1
Entrez