Smad4 Antibody

Images

 
Western Blot: SMAD4 Antibody [NBP1-51906] - Western blot analysis of SMAD4 in NIH-3T3 lysate using NBP1-51906 at 0.5 ug/ ml.
Western Blot: SMAD4 Antibody [NBP1-51906] - (0.5ug/ml) of NIH-3T3 cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Product Discontinued
View other related Smad4 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51906
    • Availability
      Product Discontinued

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Smad4 Antibody Summary

Immunogen
Peptide with sequence C-HTMPIADPQPLD corresponding to C-Terminus according to NP_005350.1.
Epitope
C-HTMPIADPQPLD
Clonality
Polyclonal
Host
Goat
Gene
SMAD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
IP: An anonymous customer has reported usage under native conditions. WB: Approx. 60-65 kDa band observed in mouse NIH-3T3 lysate.
Publications
Read Publication using NBP1-51906.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Rat, Canine, Porcine Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Smad4 Antibody

  • DPC4
  • DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
  • hSMAD4
  • JIP
  • MAD homolog 4
  • MADH4
  • MADH4mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic, Drosophila, homolog of, 4
  • SMAD 4
  • SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
  • SMAD, mothers against DPP homolog 4
  • Smad4

Background

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad4 Antibody (NBP1-51906)(1)

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMAD4
Entrez
Uniprot