Smad4 Antibody


Western Blot: SMAD4 Antibody [NBP1-31377] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SMAD4 Antibody [NBP1-31377] - Analysis of HeLa, using NBP1-31377 at 1:200 dilution.
Immunohistochemistry-Paraffin: SMAD4 Antibody [NBP1-31377] - Paraffin-embedded human ulcerative colitis tissue using SMAD4 antibody.
Western Blot: SMAD4 Antibody [NBP1-31377] - Sample (50 ug of whole cell lysate) A: mouse muscle 7. 5% SDS PAGE; antibody diluted at 1:1000.

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Smad4 Antibody Summary

Recombinant protein encompassing a sequence within the C-terminus region of human SMAD4. The exact sequence is proprietary.
Cytoplasm, Nucleus
Predicted Species
Porcine (100%), Sheep (100%), Monkey (100%), Bovine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Sheep (100%), Rhesus Monkey (100%).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.0) and 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified

Alternate Names for Smad4 Antibody

  • DPC4
  • DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
  • hSMAD4
  • JIP
  • MAD homolog 4
  • MADH4
  • MADH4mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic, Drosophila, homolog of, 4
  • SMAD 4
  • SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
  • SMAD, mothers against DPP homolog 4
  • Smad4


Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol SMAD4