Smad4 Antibody

Images

 
Western Blot: SMAD4 Antibody [NBP1-31377] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SMAD4 Antibody [NBP1-31377] - Analysis of HeLa, using NBP1-31377 at 1:200 dilution.
Immunohistochemistry-Paraffin: SMAD4 Antibody [NBP1-31377] - Paraffin-embedded human ulcerative colitis tissue using SMAD4 antibody.
Western Blot: SMAD4 Antibody [NBP1-31377] - Sample (50 ug of whole cell lysate) A: mouse muscle 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Product Discontinued
View other related Smad4 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-31377
    • Availability
      Product Discontinued

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Smad4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SMAD4. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Porcine (100%), Monkey (100%), Sheep (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Sheep (100%), Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.55 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Smad4 Antibody

  • DPC4
  • DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
  • hSMAD4
  • JIP
  • MAD homolog 4
  • MADH4
  • MADH4mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic, Drosophila, homolog of, 4
  • SMAD 4
  • SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
  • SMAD, mothers against DPP homolog 4
  • Smad4

Background

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMAD4
Entrez