Smad3 Antibody



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Smad3 Antibody Summary

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein.
A BLAST analysis was used to suggest cross-reactivity with Smad3 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. Also, the antibody is Smad3 specific, and reactivity to other Smad proteins (specifically Smad1, Smad2, Smad4, and Smad7) is not detected in over-expressed cell lysates (Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD).
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
20mM Sodium Phosphate (pH 7.2) and 0.15M NaCl
0.01% Sodium Azide
Immunogen affinity purified


Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Expect a band approximately 50 kDa in size corresponding - Smad3 protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive in human, mouse, rat


Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-b (transforming growth factor) and activin type I receptor kinase.  These activators exert diverse effects on a wide array of cellular processes.  The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activin type I receptor kinase phosphorylates receptor-activated Smads (R-Smads) at the two extreme serines in the C-terminal SSXS motif; e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the Bone Morphogenetic Proteins (BMP) pathway.  The phosphorylated R-Smad then translocates into the nucleus, where it regulates transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated responses.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Smad3 Antibody (NBP1-77846). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
    • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

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Gene Symbol SMAD3

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