Smad3 Antibody


Western Blot: Smad3 Antibody [NBP1-77846] - 231 cells (lane 2). Lane 1 shows mock infection of 231 cells with lentiviral vector alone. The membrane was probed with the primary antibody at a 1:5,000 dilution. Personal more

Product Details

Product Discontinued
View other related Smad3 Primary Antibodies

Order Details

    • Catalog Number
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Smad3 Antibody Summary

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein.
A BLAST analysis was used to suggest cross-reactivity with Smad3 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. Also, the antibody is Smad3 specific, and reactivity to other Smad proteins (specifically Smad1, Smad2, Smad4, and Smad7) is not detected in over-expressed cell lysates (Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD).
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:10000
  • ELISA 1:15000-1:50000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Expect a band approximately 50 kDa in size corresponding - Smad3 protein by western blotting in the appropriate cell lysate or extract.
Positive Control
Smad3 Lysate (NBP2-64929)

Reactivity Notes

Reactive in human, mouse, rat

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
1.06 mg/ml
Immunogen affinity purified

Alternate Names for Smad3 Antibody

  • DKFZp586N0721
  • DKFZp686J10186
  • hMAD-3
  • hSMAD3
  • HsT17436
  • JV15-2MGC60396
  • MAD homolog 3
  • mad protein homolog
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • MAD, mothers against decapentaplegic homolog 3
  • mad3
  • MADH3mad homolog JV15-2
  • mothers against decapentaplegic homolog 3
  • Mothers against DPP homolog 3
  • SMA- and MAD-related protein 3
  • SMAD 3
  • SMAD family member 3HSPC193
  • SMAD, mothers against DPP homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3
  • Smad3


Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-b (transforming growth factor) and activin type I receptor kinase.  These activators exert diverse effects on a wide array of cellular processes.  The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activin type I receptor kinase phosphorylates receptor-activated Smads (R-Smads) at the two extreme serines in the C-terminal SSXS motif; e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the Bone Morphogenetic Proteins (BMP) pathway.  The phosphorylated R-Smad then translocates into the nucleus, where it regulates transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated responses.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
Species: Hu
Applications: WB, ChIP, ICC
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
Species: Hu
Applications: WB, ELISA, ICC
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
Species: Hu, Mu, Bv, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: IHC
Species: Hu, Ba
Applications: WB, ELISA
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC

Publications for Smad3 Antibody (NBP1-77846) (0)

There are no publications for Smad3 Antibody (NBP1-77846).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Smad3 Antibody (NBP1-77846) (0)

There are no reviews for Smad3 Antibody (NBP1-77846). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Smad3 Antibody (NBP1-77846). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
    • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional Smad3 Products

Bioinformatics Tool for Smad3 Antibody (NBP1-77846)

Discover related pathways, diseases and genes to Smad3 Antibody (NBP1-77846). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Smad3 Antibody (NBP1-77846)

Discover more about diseases related to Smad3 Antibody (NBP1-77846).

Pathways for Smad3 Antibody (NBP1-77846)

View related products by pathway.

PTMs for Smad3 Antibody (NBP1-77846)

Learn more about PTMs related to Smad3 Antibody (NBP1-77846).

Research Areas for Smad3 Antibody (NBP1-77846)

Find related products by research area.

Blogs on Smad3.

TGF-beta for treating degenerative intervertebral disc disease
By Jamshed Arslan Pharm.D. Our upright posture and balance depend on a jelly-like material, called nucleus pulposus (NP), in the middle of intervertebral discs. NP cells protect us from disc degeneration by maintain...  Read full blog post.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Smad3 Antibody and receive a gift card or discount.


Gene Symbol SMAD3