Smad3 Antibody


Western Blot: SMAD3 Antibody [NBP1-01009] - Western blot (WB) analysis of SMAD3 (N175) antibody in extracts from HeLa cell

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Smad3 Antibody Summary

Synthetic peptide, corresponding to amino acids 145-190 of Human Smad3.
SMAD3 (N175) detects endogenous levels of Smad3 protein.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of SMAD3 (N175) pAb in extracts from HeLa cell.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Smad3 Lysate (NBP2-64929)

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Smad3 Antibody

  • DKFZp586N0721
  • DKFZp686J10186
  • hMAD-3
  • hSMAD3
  • HsT17436
  • JV15-2MGC60396
  • MAD homolog 3
  • mad protein homolog
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • MAD, mothers against decapentaplegic homolog 3
  • mad3
  • MADH3mad homolog JV15-2
  • mothers against decapentaplegic homolog 3
  • Mothers against DPP homolog 3
  • SMA- and MAD-related protein 3
  • SMAD 3
  • SMAD family member 3HSPC193
  • SMAD, mothers against DPP homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3
  • Smad3


Smad proteins, the mammalian homologs of the Drosophilamothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP-2 and BMP-4 function while Smad2 (also designated Madr2 or JV18-1) and SMAD3 are involved in TGFbeta and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Smad3 Antibody (NBP1-01009). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
    • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol SMAD3