Smad1 Antibody


Western Blot: SMAD1 Antibody [NBP1-51288] - Analysis of Smad1 (P457) pAb in extracts from COS7 cells.
Immunohistochemistry-Paraffin: SMAD1 Antibody [NBP1-51288] - Analyzes of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
1.0 mg/ml

Order Details

Smad1 Antibody Summary

Synthetic peptide, corresponding to amino acids 424-478 of Human Smad1.
Smad1 (P457) pAb detects endogenous levels of Smad1 protein.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate buffered saline (PBS) approx. pH 7.2.
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
Western blot (WB) analysis of Smad1 (P457) pAb in extracts from COS7 cells.
Immunohistochemistry (IHC) analyzes of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa

Reactivity Notes

Human, Mouse, Rat

Alternate Names for Smad1 Antibody

  • BSP1
  • BSP-1
  • hSMAD1
  • JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
  • MAD homolog 1
  • MADH1JV41
  • MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
  • Mad-related protein 1
  • mothers against decapentaplegic homolog 1
  • Mothers against DPP homolog 1
  • SMAD family member 1SMAD 1
  • SMAD, mothers against DPP homolog 1
  • Smad1
  • TGF-beta signaling protein 1
  • transforming growth factor-beta signaling protein 1


Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGF beta /BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF beta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGF beta signaling by interfering with TGF beta -mediated phosphorylation of other Smad family members.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


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Contact Information

Product PDFs


Gene Symbol SMAD1

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