SMAC/Diablo Antibody

Images

 
Western Blot: SMAC/Diablo Antibody [NB100-55900] - Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide (2409P) with Smac antibody at 1 ug/mL.
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NB100-55900] - Immunohistochemistry of Smac/DIABLO in human ovary tissue with Smac antibody at 5 ug/mL.
Western Blot: SMAC/Diablo Antibody [NB100-55900] - Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide (2409P) with anti-Smac using NB100-55900 at 1 ug /ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SMAC/Diablo Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Smac. The immunogen is located within the last 50 amino acids of Smac.
Specificity
Rabbit Polyclonal to Smac
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIABLO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Heart Lysate (NB820-59217)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SMAC/Diablo Antibody

  • 0610041G12Rik
  • Diablo
  • diablo, IAP-binding mitochondrial protein
  • Direct IAP-binding protein with low pI
  • mitochondrial Smac protein
  • Second mitochondria-derived activator of caspase
  • SMAC
  • SMACmitochondrial

Background

The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SMAC/Diablo Antibody (NB100-55900)

Discover related pathways, diseases and genes to SMAC/Diablo Antibody (NB100-55900). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for SMAC/Diablo Antibody (NB100-55900)

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Blogs on SMAC/Diablo.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were...  Read full blog post.

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Bioinformatics

Gene Symbol DIABLO
Entrez