SLAM/CD150 Antibody (9D1)

Images

 
Flow Cytometry: SLAM/CD150 Antibody (9D1) [NB100-63446] - Analysis using the FITC conjugate of NB100-63446. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.5 ug of Rat IgG1 kappa Isotype ...read more
Flow Cytometry: SLAM/CD150 Antibody (9D1) [NB100-63446] - Staining of mouse thymus cells.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
9D1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SLAM/CD150 Antibody (9D1) Summary

Immunogen
CHO cells stably transfected with mouse CD150
Localization
Cell membrane; single pass type I membrane protein. Present on the surface of B cells and T cells.
Specificity
NB100-63446 recognizes murine CD150, also known as signalling lymphocyte activation molecule (SLAM). Murine CD150 is a 75kDa cell surface glycoprotein that is expressed on T-cells and B-cells, and has been shown to be upregulated on activated T-cells and stimulated macrophages. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T-cells and B-cells. Clone 9D1 is reported to have functional activity (1). This antibody is routinely tested on mouse thymocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
SLAMF1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:25-1:100

Reactivity Notes

Reacts with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for SLAM/CD150 Antibody (9D1)

  • CD 150
  • CD150
  • CD150IPO-3
  • CDw150
  • IPO-3
  • signaling lymphocytic activation molecule family member 1
  • signaling lymphocytic activation molecule
  • SLAM
  • SLAMCD150 antigen
  • SLAMF1

Background

CD150, a novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) or CDw150, belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and the expression levels of membrane bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-63446AF405
Alexa Fluor 488 Labeled NB100-63446AF488
Alexa Fluor 647 Labeled NB100-63446AF647
Alexa Fluor 700 Labeled NB100-63446AF700
Allophycocyanin Labeled NB100-63446APC
DyLight 350 Labeled NB100-63446UV
DyLight 405 Labeled NB100-63446V
DyLight 488 Labeled NB100-63446G
DyLight 680 Labeled NB100-63446FR
DyLight 755 Labeled NB100-63446IR
PE Labeled NB100-63446PE
PerCP Labeled NB100-63446PCP

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Bioinformatics

Gene Symbol SLAMF1
Entrez