Skp2 Antibody

Images

 
Western Blot: Skp2 Antibody [NB100-91962] - Analysis of Skp2 p45 (P399) pAb at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:Mouse brain tissue lysateLane3:Rat brain tissue lysate
Immunohistochemistry-Paraffin: Skp2 Antibody [NB100-91962] - Analyzes of Skp2 p45 (P399) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: Skp2 Antibody [NB100-91962] - Analysis of SKP2/p45 (P399) antibody in extracts from 293 cells.
Immunohistochemistry-Paraffin: Skp2 Antibody [NB100-91962] - Paraffin-embedded human lung carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related Skp2 Primary Antibodies

Order Details


    • Catalog Number
      NB100-91962
    • Availability
      Product Discontinued

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Skp2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 369-419 of Human Skp2 p45.
Clonality
Polyclonal
Host
Rabbit
Gene
SKP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of SKP2/p45 (P399) pAb in extracts from 293 cells.
Immunohistochemistry (IHC) analyzes of SKP2/p45 (P399) pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Skp2 Antibody

  • CDK2/cyclin A-associated protein p45
  • cyclin A/CDK2-associated protein p45
  • Cyclin-A/CDK2-associated protein p45
  • FBL1
  • F-box protein Skp2
  • F-box/LRR-repeat protein 1
  • FBXL1
  • FBXL1MGC1366
  • FLB1
  • p45skp2
  • Skp2
  • S-phase kinase-associated protein 2 (p45)
  • S-phase kinase-associated protein 2

Background

The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase 2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F, and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SKP2
Entrez