Skp1 Antibody

Product Discontinued

Skp1 Antibody Summary

• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH.
• Specificity: This product reacts with human SKP1. Cross reactivity is expected against mouse SKP1 based on a high degree of sequence identity.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SKP1
• Purity: Sterile filtered

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: The antibody immunoprecipitates in vitro translated protein and protein from cell lysates . Coimmunoprecipitation of cyclin A and Cuol1 may occur. An 18.5 kDa band corresponding - human SKP1 is detected. Most cell lines expressing SKP1 can be used as a positive control. Researchers should determine optimal titers for other applications.

Reactivity Notes

Cross reactivity with Skp1 from other sources has not been tested.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: 0.01% Sodium Azide
• Concentration: 85 mg/ml
• Purity: Sterile filtered

Alternate Names for Skp1 Antibody

• Cyclin-A/CDK2-associated protein p19
• EMC19p19skp1
• MGC34403
• OCP2
• OCP2OCP-2
• OCP-IIS-phase kinase-associated protein 1A (p19A)
• Organ of Corti protein 2
• Organ of Corti protein II
• p19a
• p19Acyclin A/CDK2-associated p19
• p19Skp1
• RNA polymerase II elongation factor-like protein OCP2
• RNA polymerase II elongation factor-like protein
• SKP1
• SKP1A
• SKP1Acyclin A/CDK2-associated protein p19
• S-phase kinase-associated protein 1
• TCEB1LSIII
• Transcription elongation factor B

Background

SKP1 is also known as S-phase kinase-associated protein 1A, Cyclin A/CDK2-associated protein p19, p19A, p19skp1, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, OCP-II protein, OCP-2, Transcription elongation factor B, and SIII. SKP1 is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, SKP1 serves as an adapter that links the F-box protein to CUL1. SKP1 interacts directly with CUL1 and F-box proteins, such as BTRC and SKP2, in the SCF complex. SKP1 also interacts with FBX29 and the cyclin A/CDK2 complex. SKP1 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8 and is also a component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SKP1
• Uniprot:
  • NC_000005.9(Human)