Skp1 Antibody

Product Discontinued

Skp1 Antibody Summary

• Immunogen: Recombinant (partial), N-terminal
• Specificity: SKP1A
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SKP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse, Human.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Skp1 Antibody

• Cyclin-A/CDK2-associated protein p19
• EMC19p19skp1
• MGC34403
• OCP2
• OCP2OCP-2
• OCP-IIS-phase kinase-associated protein 1A (p19A)
• Organ of Corti protein 2
• Organ of Corti protein II
• p19a
• p19Acyclin A/CDK2-associated p19
• p19Skp1
• RNA polymerase II elongation factor-like protein OCP2
• RNA polymerase II elongation factor-like protein
• SKP1
• SKP1A
• SKP1Acyclin A/CDK2-associated protein p19
• S-phase kinase-associated protein 1
• TCEB1LSIII
• Transcription elongation factor B

Background

SKP1A (S-phase kinase-associated protein 1A) is an 18 kD protein found in the nucleus an the cytoplasm. This protein contains an F-box and has two different isoforms with slightly differnt molecular weights (A, 18.1 kD and B, 18.6 kD). SKP1A functions as a substrate recognition component of SCF ubiquitin ligase complex and RNA polymerase II elongation factor. SKP1A has also been shown to collaborate with a network of proteins to control beta-catenin levels and affect beta-catenin dependent TCF transcription factors. SKP1A can be modified by glycosylation and ubquitination. This protein has been shown to interact with cyclin F, Skp2, CUL1, cyclin A, and CDK2. The Poly6032 antibody recognizes the N-terminal human and mouse SKP1A protein and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Skp1 Antibody (NB100-78268)(1)

Publication using NB100-78268

• Pilar AV, Reid-Yu SA, Cooper CA et al. GogB is an anti-inflammatory effector that limits tissue damage during Salmonella infection through interaction with human FBXO22 and Skp1. PLoS Pathog 2012-01-01 [PMID: 22761574] (WB, Mouse)

Bioinformatics

• Gene Symbol: SKP1
• Entrez:
  • 21402(Mouse)