Sirtuin 1/SIRT1 Antibody

Images

 
Western Blot: SIRT1 Antibody [NBP1-72060] - (0.05ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SIRT1 Antibody [NBP1-72060] - (3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-72060
    • Availability
      Product Discontinued

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Sirtuin 1/SIRT1 Antibody Summary

Immunogen
Peptide with sequence C-EKPQEVQTSRNVES corresponding to internal region according to NP_036370.2, NP_001135970.1.
Epitope
CEKPQEVQTSRNVES
Specificity
This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
Clonality
Polyclonal
Host
Goat
Gene
SIRT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05 - 0.2 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 120 kDa band observed in Jurkat lysates is reported in scientific literature (PMID: 12960381). IHC-P: Human skin shows perinuclear staining in select keratinocytes.
Publications
Read Publications using NBP1-72060.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Sirtuin 1/SIRT1 Antibody

  • EC 3.5.1
  • S. cerevisiae, homolog) 1
  • SIR2alpha
  • SIR2L1
  • SIR2-like protein 1
  • SIRT1
  • Sirtuin 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sirtuin 1/SIRT1 Antibody (NBP1-72060)(2)

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Video Protocols

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Bioinformatics Tool for Sirtuin 1/SIRT1 Antibody (NBP1-72060)

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Bioinformatics

Gene Symbol SIRT1
Entrez
Uniprot