Sirtuin 1/SIRT1 Antibody

Images

 
Western Blot: Sirtuin 1/SIRT1 Antibody [NB100-53785] - (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Sirtuin 1/SIRT1 Antibody [NB100-53785] - (1ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-53785
    • Availability
      Product Discontinued

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Sirtuin 1/SIRT1 Antibody Summary

Immunogen
Peptide with sequence C-EITEKPPRTQKE corresponding to internal region according to NP_036370.2.
Clonality
Polyclonal
Host
Goat
Gene
SIRT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 120 kDa band observed in mouse fetal brain lysates is reported in scientific literature (PMID: 12960381).
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publication using NB100-53785.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Sirtuin 1/SIRT1 Antibody

  • EC 3.5.1
  • S. cerevisiae, homolog) 1
  • SIR2alpha
  • SIR2L1
  • SIR2-like protein 1
  • SIRT1
  • Sirtuin 1

Background

Sirt1, an HDAC human homologue of the yeast Sir2 protein important in establishing repressive chromatin structures, has been found to associate with the tumor suppressor protein p53. It binds and deacetylates p53 in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress. It has also been shpwn to play a role in increasing lifespan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sirtuin 1/SIRT1 Antibody (NB100-53785)(1)


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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Sirtuin 1/SIRT1 Antibody (NB100-53785)

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Bioinformatics

Gene Symbol SIRT1
Entrez
Uniprot