Sirtuin 1/SIRT1 Antibody (E104)

Product Discontinued

Sirtuin 1/SIRT1 Antibody (E104) Summary

• Immunogen: A synthetic peptide corresponding to residues in C-terminus of human Sir2/SIRT1 was used as immunogen.
• Specificity: The antibody does not cross-react with other sirtuin family members.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SIRT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-10000
• Application Notes: In Western blot this antibody detects a band at approximately 110 kDa. Use in IHC-F was reported in the scientific literature (PMID: 21098725).
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A or G purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Sirtuin 1/SIRT1 Antibody (E104)

• EC 3.5.1
• S. cerevisiae, homolog) 1
• SIR2alpha
• SIR2L1
• SIR2-like protein 1
• SIRT1
• Sirtuin 1

Background

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+ -dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2, SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sirtuin 1/SIRT1 Antibody (NB110-57573)(6)

Publications using NB110-57573

• Jin SH, Yang JH, Shin BY et al. Resveratrol inhibits LXRalpha-dependent hepatic lipogenesis through novel antioxidant Sestrin2 gene induction. Toxicol Appl Pharmacol 2013-05-04 [PMID: 23651738] (WB, Human)
• Sung JY, Kim R, Kim JE et al. Balance between SIRT1 and DBC1 expression is lost in breast cancer. Cancer Sci. 2010-07-01 [PMID: 20412117]
• Gracia-Sancho J, Villarreal G Jr, Zhang Y et al. Activation of SIRT1 by resveratrol induces KLF2 expression conferring an endothelial vasoprotective phenotype. Cardiovasc Res. 2010-02-01 [PMID: 19815564]
• Firestein R, Blander G, Michan S et al. The SIRT1 deacetylase suppresses intestinal tumorigenesis and colon cancer growth. PLoS One 2008-04-01 [PMID: 18414679] (IF/IHC, ICC/IF)
• Uno A, Hitosugi M, Nakamura M et al. Complement C9 expression is associated with damaged myocardial cells in pediatric sudden death cases of fulminant myocarditis Egypt J Forensic Sci 2020-12-01 (IHC-P, Human)
  
  Details:
  Human heart samples were analyzed by immunohistochemistry.
• Bhalla K, Jaber S, Reagan K et al. SIRT3, a metabolic target linked to ataxia-telangiectasia mutated (ATM) gene deficiency in diffuse large B-cell lymphoma Scientific reports 2020-12-03 [PMID: 33273545] (IF/IHC, Human)
  
  Details:
  Several cancerous tissues and cells underwent immunohistochemical analysis.

Bioinformatics

• Gene Symbol: SIRT1
• Entrez:
  • 23411(Human)
  • 93759(Mouse)
  • 309757(Rat)
• Uniprot:
  • Q96EB6(Human)