SIRT6 Antibody

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Western Blot: SIRT6 Antibody [NBP1-30101] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit antiSirt6 antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SIRT6 Antibody Summary

Immunogen
Synthetic peptide mapping to a region between residue 305 and 355 of human sirtuin (silent mating type information regulation 2 homolog) 6 using the numbering given in entry NP_057623.1 (GeneID 51548).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRT6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP1-30101 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIRT6 Antibody

  • EC 3.5.1.-
  • NAD-dependent deacetylase sirtuin-6
  • SIR2L6sir2-related protein type 6
  • SIR2-like protein 6
  • sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
  • sirtuin 6
  • sirtuin type 6

Background

The SIRT6 is included in class IV of the sirtuin family, homologs to the yeast Sir2 protein. The functions of human sirtuins have not yet been determined; however, in yeast sirtuins are known to regulate epigenetic gene silencing. Also SIRT6, a chromatin-associated protein, appears to be involved in DNA repair. Recombinant SIRT6, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for SIRT6 Antibody (NBP1-30101) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-30101:
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Images Ratings Applications Species Date Details
Western Blot SIRT6 NBP1-30101
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reviewed by:
Anonymous
WB Human 05/15/2017
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Summary

ApplicationWestern Blot
Sample TestedCaco-2 whole cell lysate
SpeciesHuman

Comments

CommentsWhole cell lysate from Caco-2 cells (4.5, 9 and 18 ug/sample) in the experiment of 24 h.

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Secondary Antibodies

 

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Recent Reviews

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Anonymous
05/15/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol SIRT6
Entrez
Uniprot