Recombinant Human Single-stranded DNA Binding Protein

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SDS-Page: Single-stranded DNA Binding Protein [NBP2-35314]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free

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Recombinant Human Single-stranded DNA Binding Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 148 residues of Human Single-stranded DNA Binding Protein.


Source: E. coli

Amino Acid Sequence: MEEKVGNLKP NMESVNVTVR VLEASEARQI QTKNGVRTIS EAIVGDETGR VKLTLWGKHA GSIKEGQVVK IENAWTTAFK GQVQLNAGSK TKIAEASEDG FPESSQIPEN TPTAPQQMRG GGRGFRGGGR RYGRRGGRRQ ENEEGEEE

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 0.1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
16.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered concentrated solution in 20 mM Tris, pH 7.4, 200 mM NaCl, 50 % Glycerol, 1 mM EDTA, 0.5 mM DTT.
Preservative
50% glycerol
Purity
>95% pure by SDS-PAGE

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Background

Single-stranded DNA-binding protein, or SSB, binds to single-stranded regions of DNA to prevent premature annealing, to protect the single-stranded DNA from being digested by nucleases, and to remove secondary structure from the DNA to allow other enzymes to function effectively upon it. In molecular biology, SSB protein domain in bacteria are important in its function of maintaining DNA metabolism, more specifically DNA replication, repair and recombination. SSB proteins have been identified in organisms from viruses to humans. The only organisms known to lack them are thermoproteales, a group of extremophile archaea, where they have been displaced by the protein ThermoDBP. While many phage and viral SSBs function as monomers and eukaryotes encode heterotrimeric RPA (Replication Protein A), the best characterized SSB is that from the bacteria E. coli which, like most bacterial SSBs exists as a tetramer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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