Siglec-2/CD22 Antibody (HIB22) [FITC]

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Flow Cytometry: Siglec-2/CD22 Antibody (HIB22) [FITC] [NB100-77771] - Human peripheral blood lymphocytes stained HIB22 FITC.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-77771
    • Availability
      Product Discontinued

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Siglec-2/CD22 Antibody (HIB22) [FITC] Summary

Immunogen
The immunogen was the full length version of the protein: CD22 (HIB22)
Specificity
CD22 (HIB22)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD22
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Expected Cross-Reactivity based on immunogen sequence: Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2) and 0.2% (w/v) BSA
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Siglec-2/CD22 Antibody (HIB22) [FITC]

  • B-cell receptor CD22
  • BL-CAM
  • B-lymphocyte cell adhesion molecule
  • CD22 antigenMGC130020
  • CD22 molecule
  • CD22
  • sialic acid binding Ig-like lectin 2
  • Sialic acid-binding Ig-like lectin 2
  • Siglec2
  • Siglec-2
  • SIGLEC2FLJ22814
  • T-cell surface antigen Leu-14

Background

CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase C gamma1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Siglec-2/CD22 Antibody (NB100-77771). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?
    • We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

Secondary Antibodies

 

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Bioinformatics Tool for Siglec-2/CD22 Antibody (NB100-77771)

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Diseases for Siglec-2/CD22 Antibody (NB100-77771)

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PTMs for Siglec-2/CD22 Antibody (NB100-77771)

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Bioinformatics

Gene Symbol CD22
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