Sialoganglioside Antibody (3G11)


Flow Cytometry: Sialoganglioside Antibody (3G11) [NBP2-00370] - Staining of BALB/c thymocytes with 0.5 ug of Mouse IgM Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse/Rat 3G11 Sialoganglioside Antigen more

Product Details

Reactivity Mu, RtSpecies Glossary
Applications Flow
0.5 mg/ml

Sialoganglioside Antibody (3G11) Summary

The immunogen for this antibody is 3G11 sialoganglioside antigen.
IgM Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous, may contain carrier protein/stabilizer.
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Flow Cytometry 1:10-1:1000
Application Notes
This 3G11 (SM3G11, 3G11) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that theantibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.


The 3G11 monoclonal antibody reacts with the mouse 3G11antigen. The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes. 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age. In thymus, 3G11 is expressed on approximately 20% of total thymocytes. 3G11 has also been used as a marker to distinguish naive and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells. The 3G11 antibody is able to block binding of antibodies to CD3e, suggesting close proximity of these two proteins.

Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype. Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP2-00370AF405
Alexa Fluor (R) 488 Labeled NBP2-00370AF488
Alexa Fluor (R) 647 Labeled NBP2-00370AF647
Alexa Fluor (R) 700 Labeled NBP2-00370AF700
Allophycocyanin Labeled NBP2-00370APC
DyLight 350 Labeled NBP2-00370UV
DyLight 405 Labeled NBP2-00370V
DyLight 405LS Labeled NBP2-00370V3
DyLight 488 Labeled NBP2-00370G
DyLight 680 Labeled NBP2-00370FR
DyLight 755 Labeled NBP2-00370IR
PE Labeled NBP2-00370PE
PerCP Labeled NBP2-00370PCP

Additional Sialoganglioside Antibody (3G11) Products

Sialoganglioside NBP2-00370

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