SHP-2/PTPN11 [p Tyr542] Antibody

Images

 
Western Blot: PTPN11/SHP2 [phospho Tyr542] Antibody [NB100-92627] - SH-PTP2 (Y580) pAb in A549 cells.
Immunohistochemistry-Paraffin: PTPN11/SHP2 [phospho Tyr542] Antibody [NB100-92627] Human brain tissue.
Western Blot: PTPN11/SHP2 [phospho Tyr542] Antibody [NB100-92627] - Western blot (WB) analysis of p-SHP-2 (Y542) antibody in extracts from A431 or HuvEc cells.

Product Details

Summary
Product Discontinued
View other related SHP-2/PTPN11 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92627
    • Availability
      Product Discontinued

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SHP-2/PTPN11 [p Tyr542] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human SHP-2 around the phosphorylation site of Tyrosine 542.
Modification
p Tyr542
Clonality
Polyclonal
Host
Rabbit
Gene
PTPN11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of p-SH-PTP2 (Y580) pAb in A549 cells.
Immunohistochemistry (IHC) analyzes of p-SH-PTP2 (Y580) pAb in paraffin-embedded human brain tissue.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SHP-2/PTPN11 [p Tyr542] Antibody

  • BPTP3
  • EC 3.1.3.48
  • MGC14433
  • protein tyrosine phosphatase, non-receptor type 11
  • protein tyrosine phosphatase-2
  • Protein-tyrosine phosphatase 1D
  • Protein-tyrosine phosphatase 2C
  • PTP1D
  • PTP-1D
  • PTP2C
  • PTP-2C
  • PTP2CNS1
  • PTPN11
  • SHP2
  • SHP-2
  • SHP2CFC
  • SHPTP2
  • SH-PTP2
  • SH-PTP2Noonan syndrome 1
  • SH-PTP3
  • tyrosine-protein phosphatase non-receptor type 11

Background

SHP (also designated short heterodimer partner and small heterodimer partner) is an orphan nuclear receptor containing the dimerization and ligandbinding domains found in other nuclear receptors but lacking the conserved DNA binding domain. SHP is specifically expressed in liver and other tissues including fetal liver and adrenal gland, adult spleen and small intestine. In addition, SHP is highy expressed in the murine macrophage cell line RAW 264.7 but suppressed by oxLDL and 13-HODE, which is a ligand for PPARgamma. SHP interacts with nuclear receptors including thyroid receptor, retinoic acid receptors (RAR and RXR) and estrogen receptors (ERa and ERb). SHP functions as a negative regulator of these receptors by at least three mechanisms: inhibition of DNA binding via dimerization, direct antagonism of coactivator function via competition and possibly transrepression via recruitment of putative corepressors. In oxLDL-treated, resting macrophage cells, SHP acts as a transcription coactivator of NF-kappaB, suggesting that SHP is a modulatory component in the regulation of the transcriptional activities of NF-kappaB. Lastly, negative feedback regulation of a hepatic bile acid transporter NTCP is controlled by bile acid-activated FXR via induction of SHP to protect the hepatocyte from bile acid-mediated damage in cholestatic conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PTPN11
Entrez