SHMT1 Overexpression Lysate (Denatured)

Images

 
Lane 1: SHMT1 transfected lysate (53.24 KDa) Lane 2: Non-transfected lysate.
SHMT1 transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SHMT1 Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-SHMT1 full-length
Gene
SHMT1

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for SHMT1

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-SHMT1 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SHMT1 Overexpression Lysate (Denatured)

  • 14 kDa protein
  • CSHMT
  • cytoplasmic serine hydroxymethyltransferase
  • Glycine hydroxymethyltransferase
  • MGC15229
  • MGC24556
  • serine hydroxymethyltransferase 1 (soluble)
  • serine hydroxymethyltransferase, cytosolic
  • Serine methylase
  • SHMTEC 2.1.2.1

Background

This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol SHMT1