SHC1 Antibody

Images

 
Western Blot: SHC1 Antibody [NB100-92055] - Analysis of SHC1 Antibody in extracts from HeLa cells treated with Calyculin A 50nM 15' or HeLa cells treated with Calyculin A 100nM 30'.
Immunohistochemistry: SHC1 Antibody [NB100-92055] - Analyzes of SHC1 Antibody in paraffin-embedded human brain colon carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related SHC1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92055
    • Availability
      Product Discontinued

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SHC1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 390-440 of Human Shc.
Specificity
SHC1 (L421) detects endogenous levels of Shc protein.
Clonality
Polyclonal
Host
Rabbit
Gene
SHC1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of SHC1 (L421) pAb in extracts from HeLa cells treated with Calyculin A 50nM 15' or HeLa cells treated with Calyculin A 100nM 30'.
Immunohistochemistry (IHC) analyzes of SHC1 (L421) pAb in paraffin-embedded human brain colon
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SHC1 Antibody

  • FLJ26504
  • p66
  • p66SHC
  • SH2 domain protein C1
  • SHC (Src homology 2 domain containing) transforming protein 1
  • SHC (Src homology 2 domain-containing) transforming protein 1
  • SHC
  • SHC1
  • SHCA
  • SHC-transforming protein 1
  • SHC-transforming protein 3
  • SHC-transforming protein A
  • Src homology 2 domain-containing-transforming protein C1

Background

Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus activating mitogenic, as well as other, responses. Critical components of this process include adapter proteins such as SHC1 and IRS-1 that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics Tool for SHC1 Antibody (NB100-92055)

Discover related pathways, diseases and genes to SHC1 Antibody (NB100-92055). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol SHC1
Entrez