SFRS3 Antibody

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Western Blot: SFRS3 Antibody [NBP1-01020] - SFRS3 Antibody Western blot (WB) analysis of SRp20 (F151) antibody in extracts from HeLa

Product Details

Summary
Product Discontinued
View other related SFRS3 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-01020
    • Availability
      Product Discontinued

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SFRS3 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 120-170 of Human SRp20.
Specificity
SRp20 (F151) detects endogenous levels of SFRS3 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
SRSF3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of SRp20 (F151) pAb in extracts from HeLa.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SFRS3 Antibody

  • Pre-mRNA-splicing factor SRP20
  • serine/arginine-rich splicing factor 3
  • SFRS3splicing factor, arginine/serine-rich, 20-kD
  • Splicing factor, arginine/serine-rich 3SRP20
  • SRp20

Background

Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns that are required for accurate splice site recognition and the control of alternative splicing. Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF/SF2 and RNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SRSF3
Entrez