Recombinant Human Serpin E1/PAI-1 His Protein

SDS-Page: Recombinant Human Serpin E1/PAI-1 Protein [NBC1-18526] - PAI1/Serpine1, 45 kDa (400 aa) with a purity of 95% by SDS - PAGE

Product Discontinued

Recombinant Human Serpin E1/PAI-1 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 24-402 of Human Serpin E1/PAI-1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP

• Details of Functionality: The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SERPINE1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50 mM NaAc (pH 5.5), 10% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Serpin E1/PAI-1 His Protein

• Endothelial plasminogen activator inhibitor
• Nexin
• PAI1
• PAI-1
• PAI1PAI-1
• PAISerpin E1
• PLANH1
• PLANH1plasminogen activator inhibitor 1
• serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
• Serpin E1
• serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

Plasminogen activator inhibitor-1(PAI1), a member of the serine protease inhibitor(serpin) super family. PAI1/Serpine1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue. Recombinant human PAI1/Serpine1, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SERPINE1