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Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

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ELISA: Human Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP2-60584] - Standard Curve
ELISA: Human Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP2-60584] - Standard Curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell Culture, Tissue Extract
Standard Curve Range
0.063-4 ng/ml (example only; lot dependent)
Sensitivity
0.04 ng/ml (example only; lot dependent)

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Human Serpin E1/PAI-1 ELISA Kit (Colorimetric) Summary

Description
This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Standard Curve Range
0.063-4 ng/ml (example only; lot dependent)
Sensitivity
0.04 ng/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 8.70% (example only; lot dependent)
Intra-Assay
CV% < 4.60% (example only; lot dependent)
Spike Recovery
98.00% (example only; lot dependent)
Kit Type
ELISA Kit (Colorimetric)
Gene
SERPINE1

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Alternate Names for Human Serpin E1/PAI-1 ELISA Kit (Colorimetric)

  • Endothelial plasminogen activator inhibitor
  • Nexin
  • PAI1
  • PAI-1
  • PAI1PAI-1
  • PAISerpin E1
  • PLANH1
  • PLANH1plasminogen activator inhibitor 1
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
  • Serpin E1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.
Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.
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t-Plasminogen
Activator/tPA Antibod ...
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Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (AH54-10) [NBP2-67279] - Staining TPA in HepG2 cells (red). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody (AH54-10) [NBP2-67279] - Analysis of paraffin-embedded mouse liver tissue using anti-TPA Tubulin antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (AH54-10) [NBP2-67279] - Staining TPA in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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TNF-alpha
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Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 &amp; 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 &amp; 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 &amp; 6). The difference is minimal in PrP-/- samples (panels 7 &amp; 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
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NBP2-23368
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Bioinformatics

Gene Symbol SERPINE1