Recombinant Human Serpin B5/Maspin Protein

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SDS-Page: Recombinant Human Serpin B5/Maspin Protein [NBP2-61991] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
0.5 mg/ml

Order Details

Recombinant Human Serpin B5/Maspin Protein Summary

Description
A Biologically active recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 20-406 of Human Serpin A8/Angiotensinogen

Source:E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHRHHPREMK KRVEDLHVGA TVAPSSRRDF TFDLYRALAS AAPSQNIFFS PVSISMSLAM LSLGAGSSTK MQILEGLGLN LQKSSEKELH RGFQQLLQEL NQPRDGFQLS LGNALFTDLV VDLQDTFVSA MKTLYLADTF PTNFRDSAGA MKQINDYVAK QTKGKIVDLL KNLDSNAVVI MVNYIFFKAK WETSFNHKGT QEQDFYVTSE TVVRVPMMSR EDQYHYLLDR NLSCRVVGVP YQGNATALFI LPSEGKMQQV ENGLSEKTLR KWLKMFKKRQ LELYLPKFSI EGSYQLEKVL PSLGISNVFT SHADLSGISN HSNIQVSEMV HKAVVEVDES GTRAAAATGT IFTFRSARLN SQRLVFNRPF LMFIVDNNIL FLGKVNRP

Details of Functionality
Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 2 nM
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SERPINB5
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
45.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM MES buffer (pH6.0) containing 150mM NaCl, 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Serpin B5/Maspin Protein

  • Maspin
  • Peptidase inhibitor 5
  • PI5
  • PI-5
  • PI5protease inhibitor 5 (maspin)
  • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
  • Serpin B5
  • serpin peptidase inhibitor, clade B (ovalbumin), member 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SERPINB5
Entrez