Home » Serpin B5/Maspin » Serpin B5/Maspin Antibodies » Serpin B5/Maspin Antibody (2N6) [DyLight 755]

Serpin B5/Maspin Antibody (2N6) [DyLight 755]

 Submit a Review

Images

 
There are currently no images for Serpin B5/Maspin Antibody (NBP1-44377IR).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2N6
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-44377AF405
Alexa Fluor 488
NBP1-44377AF488
Alexa Fluor 647
NBP1-44377AF647
Alexa Fluor 700
NBP1-44377AF700
DyLight 350
NBP1-44377UV
DyLight 405
NBP1-44377V
DyLight 488
NBP1-44377G
DyLight 680
NBP1-44377FR
Unconjugated
NBP1-44377
100% Guarantee on every product

Serpin B5/Maspin Antibody (2N6) [DyLight 755] Summary

Immunogen
mbinant Maspin (1-375aa) purified from E. coli
Specificity
This antibody is specific to MASPIN
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SERPINB5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence

Reactivity Notes

This antibody is reactive in Human

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Serpin B5/Maspin Antibody (2N6) [DyLight 755]

  • Maspin
  • Peptidase inhibitor 5
  • PI5
  • PI-5
  • PI5protease inhibitor 5 (maspin)
  • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
  • Serpin B5
  • serpin peptidase inhibitor, clade B (ovalbumin), member 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(2 Reviews)
(22 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
u-Plasminogen
Activator/Urokinase A ...
AF1310
u-Plasminogen Activator (uPA)/Urokinase was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human u-Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
u-Plasminogen Activator (uPA)/Urokinase was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human u-Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(4 Publications)
Species: Hu
Applications: WB, IHC, IP
EGFR [p Tyr1173]
Antibody [Unconjugate ...
AF1095
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated(‑) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y1173) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EGFR phosphorylated at Y1173 was detected in immersion fixed frozen sections of mouse embryo using Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1095) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
EGFR phosphorylated at Y1173 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1095) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(5 Publications)
Species: Hu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICC, ICFlow
p63/TP73L Antibody
[Unconjugated]
AF1916
p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(2 Reviews)
Species: Hu
Applications: WB, IHC, ICC
p21/CIP1/CDKN1A
Antibody (WA-1 (HJ21)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(10 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
CKAP4/p63 Antibody
NBP1-26642
Western Blot: CKAP4/p63 Antibody [NBP1-26642] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies NBP1-26641and NBP1-26643.
Immunocytochemistry/Immunofluorescence: CKAP4/p63 Antibody [NBP1-26642] - FFPE section of human placenta. Antibody used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-26642] - Human breast carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).
(1 Publication)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Serpin B3/SCCA1
Antibody (1A12)
NBP2-45788
Western Blot: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Serpin B3/SCCA1.
Immunohistochemistry: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Species: Hu
Applications: WB, IHC
SerpinB2 Antibody
(930109) [Unconjugate ...
MAB8550
Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin B2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line treated with PMA but is not detected in Serpin B2 knockout (KO) K562 cell line using Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Serpin B2 was detected in immersion fixed U937 human histiocytic lymphoma cell line stimulated with PMA using Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB8550) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC, KO
Ovalbumin Antibody
NB600-922
Western Blot: Ovalbumin Antibody [NB600-922] - Lane 1: Reduced Ovalbumin. Load: 50 ng. Primary antibody: Ovalbumin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin.
Western Blot: Ovalbumin Antibody [NB600-922] - Western blot detection of Ovalbumin from hen egg white using NB600-922 at 1:10,000. The blot was blocked ON at 4 degrees C in 3% BSA. The secondary antibody used was a DyLight 649 Conjugated Goat anti-rabbit IgG (H&L) diluted 1:20,000 and incubated 1 hr RT in blocking buffer for fluorescent western blotting.
Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Dilution: 1:1,000. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.
(4 Publications)
Species: Ch, Vi
Applications: WB, ELISA, IHC, IHC-P, IP
ER alpha/NR3A1
Antibody (EVG F9)
NB100-74413
Western Blot: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] - Analysis of various whole cell lysates.
Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] - Analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) shows staining in U251 Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] - Cancer biopsies of deparaffinized Human breast carcinoma tissues.
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
SERPINB4 Antibody
(4C8)
NBP2-03595
Western Blot: SERPINB4 Antibody (4C8) [NBP2-03595] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB4.
Immunohistochemistry-Paraffin: SERPINB4 Antibody (4C8) [NBP2-03595] Staining of paraffin-embedded Human liver tissue using anti-SERPINB4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SERPINB4 Antibody (4C8) [NBP2-03595] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SERPINB4 mouse monoclonal antibody.
Species: Hu
Applications: WB, IHC, IHC-P
UVRAG Antibody
NBP2-24482
Western Blot: UVRAG Antibody [NBP2-24482] - Analysis of UVRAG in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart using this antibody at 0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB
RPE65 Antibody
(401.8B11.3D9)
NB100-355
Western Blot: RPE65 Antibody (401.8B11.3D9) [NB100-355] - WB analysis of RPE65 in 20ug lysate of COS7 cells expressing recombinant Human RPE65 (Lane 1) and 5ug of Bovine retinal pigment epithelium membrane fraction (Lane 2). Blot processed for detection with alkaline phosphatase conjugated goat-anti Mouse IgG secondary antibody and NBT/BCIP substrate.
Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.
Immunohistochemistry-Frozen: RPE65 Antibody (401.8B11.3D9) [NB100-355] - Staining of RPE65 in a cryosection of mouse retina tissue using RPE65 antibody (clone 401.8B11.3D9).
(5 Reviews)
(41 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Xp
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
PRAP1 Antibody
NBP1-80633
Western Blot: PRAP1 Antibody [NBP1-80633] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: PRAP1 Antibody [NBP1-80633] - Staining of human cell line Hep G2 shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: PRAP1 Antibody [NBP1-80633] - Staining of human duodenum shows strong positivity in a subset of cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Mammaglobin A
Antibody (3C8)
NBP1-51671
Western Blot: Mammaglobin A Antibody (3C8) [NBP1-51671] - Western blot analysis using SCGB2A2 mouse mAb against SCGB2A2 recombinant protein (1).
Immunocytochemistry/Immunofluorescence: Mammaglobin A Antibody (3C8) [NBP1-51671] - Immunofluorescence analysis of Hela cells using SCGB2A2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Mammaglobin A Antibody (3C8) [NBP1-51671] - Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using SCGB2A2 mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Androgen R/NR3C4
[p Ser213, p Ser210] ...
NB100-56603
Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi
(12 Publications)
Species: Hu, Pm
Applications: WB, IHC, IHC-P
Thrombospondin-1
Antibody (A6.1)
NB100-2059
Western Blot: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Human Cells
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Human placenta.
Western Blot: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Human Cells
(4 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Sh
Applications: WB, B/N, ICC/IF, IHC, IHC-P, IP
Serpin B5/Maspin
Antibody (2N6) [DyLig ...
NBP1-44377IR
Serpin B5/Maspin Antibody (2N6) [DyLight 755]
Species: Hu
Applications: WB, ELISA, ICC/IF

Publications for Serpin B5/Maspin Antibody (NBP1-44377IR) (0)

There are no publications for Serpin B5/Maspin Antibody (NBP1-44377IR).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Serpin B5/Maspin Antibody (NBP1-44377IR) (0)

There are no reviews for Serpin B5/Maspin Antibody (NBP1-44377IR). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Serpin B5/Maspin Antibody (NBP1-44377IR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NBP1-44377UV
DyLight 405 Labeled NBP1-44377V
DyLight 488 Labeled NBP1-44377G
DyLight 680 Labeled NBP1-44377FR
Learn about Custom Antibody Labeling

Additional Serpin B5/Maspin Products

Related Products by Gene

AR, CDKN1A, CKAP4, EGFR, ERBB2, ESR1, OVALBUMIN, PLAU, PLAUR, PRAP1, RPE65, SCGB2A2, SERPINB2, SERPINB3, SERPINB4, THBS1, TP53, TP63, UVRAG, VEGFA

Bioinformatics Tool for Serpin B5/Maspin Antibody (NBP1-44377IR)

Discover related pathways, diseases and genes to Serpin B5/Maspin Antibody (NBP1-44377IR). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Serpin B5/Maspin Antibody (NBP1-44377IR)

Discover more about diseases related to Serpin B5/Maspin Antibody (NBP1-44377IR).
 

Pathways for Serpin B5/Maspin Antibody (NBP1-44377IR)

View related products by pathway.

PTMs for Serpin B5/Maspin Antibody (NBP1-44377IR)

Learn more about PTMs related to Serpin B5/Maspin Antibody (NBP1-44377IR).
 

Research Areas for Serpin B5/Maspin Antibody (NBP1-44377IR)

Find related products by research area.

Blogs on Serpin B5/Maspin

There are no specific blogs for Serpin B5/Maspin, but you can read our latest blog posts.

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Serpin B5/Maspin Antibody (2N6) [DyLight 755] and receive a gift card or discount.


Bioinformatics

Gene Symbol SERPINB5
Entrez

Customers Who Bought This Also Bought

Mouse IgG2b Isotype Control (MPC-11) [DyLight 755]
NBP2-27231IR
Serpin B5/Maspin Antibody (2N6) [DyLight 350]
NBP1-44377UV
Recombinant Human Serpin B5/Maspin Protein
NBP2-34953-5ug
Serpin B5/Maspin Blocking Peptide
NB110-97377PEP