Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

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ELISA: Serpin A1/alpha 1-Antitrypsin ELISA Kit [Biotin] [KA0458] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Plasma, Serum
Standard Curve Range
0.039 to 40 ug/mL
Sensitivity
0.018 ug/mL

Order Details

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.039 to 40 ug/mL
Sensitivity
0.018 ug/mL
Assay Type
Competitive ELISA
Inter-Assay
CV% < 7.10%
Intra-Assay
CV% < 4.20%
Spike Recovery
98.50%
Sample Volume
25 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SERPINA1

Applications/Dilutions

Application Notes
SERPINA1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human SERPINA1 in plasma and serum.
Publications
Read Publications using KA0458.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Human alpha-1 Antitrypsin: lyophilized.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. Human alpha-1 Antitrypsin Microplate: A 96-well polystyrene microplate (12 strips of 8wells) coated with a polyclonal antibody against human A1AT.
  4. Human alpha-1 Antitrypsin Standard: Human A1AT in a buffered protein base,lyophilized.
  5. MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  6. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  7. Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of theindividual assay.
  8. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.
  9. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

  • A1A
  • A1AT
  • AATMGC23330
  • alpha 1-Antitrypsin
  • alpha 1-Proteinase Inhibitor
  • Alpha-1 protease inhibitor
  • Alpha-1-antiproteinase
  • alpha-1-antitrypsin
  • antitrypsin), member 1
  • member 1
  • PIMGC9222
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • serine (or cysteine) proteinase inhibitor, clade A, member 1
  • Serpin A1
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Serpin A1/alpha 1-Antitrypsin ELISA Kit (KA0458)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Serpin A1/alpha 1-Antitrypsin ELISA Kit (KA0458). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ELISA kit to measure A1AT in sputum samples treated with dithiothreitol (DTT). I found this kit on your website (cat no KA0458) and just wondered if you have any data or experience using it in sputum samples?
    • KA0458 is a product we distribute for Abnova and I have inquired with them regarding the sputum samples. I was informed that they have not tested this kit with sputum samples, and therefore cannot offer any advice on this sample type specifically and do not know how well it would work with this kit.

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Bioinformatics

Gene Symbol SERPINA1
Entrez