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Semaphorin 4D/CD100 Antibody (133.1G6) [DyLight 350]

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-42504UV
    • Availability
      Product Discontinued

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Semaphorin 4D/CD100 Antibody (133.1G6) [DyLight 350] Summary

Immunogen
The antibody reacts with the CD100 antigen.
Specificity
133.1G6 reacts with human CD100, a novel 150 kD homodimer cell-surface antigen that is expressed on resting and PHA-stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor T cell lines. The mAb was clustered at the IV WLDA (workshop no. A022). Source: A BALB/c mouse was immunized with PHA stimulated PBL. Splenocytes were fused with mouse myeloma NS1 cells.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
SEMA4D
Purity
IgM purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry

Reactivity Notes

Human,Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
IgM purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Semaphorin 4D/CD100 Antibody (133.1G6) [DyLight 350]

  • A8
  • BB18
  • CD100 antigen
  • CD100
  • CD100M-sema-G
  • COLL-4
  • FLJ33485
  • FLJ34282
  • FLJ39737
  • FLJ46484
  • immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
  • MGC169138
  • MGC169141
  • M-sema-G
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
  • SEMA4D
  • SEMAJ
  • Semaphorin 4D
  • semaphorin-4D

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 20 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Proximity Ligation Assay: RFC1 Antibody [NB600-961] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human RFC1 (NB600-961). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: RFC1 Antibody [NB600-961] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human RFC1 (NB600-961). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
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Western Blot: RFC4 Antibody [NBP2-48927] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG
Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-48927] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
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PTK9 Antibody
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Western Blot: PTK9 Antibody [NBP1-90297] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: PTK9 Antibody [NBP1-90297] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
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alpha-Sarcoglycan
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Western Blot: alpha Sarcoglycan Antibody [NBP1-90306] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: alpha Sarcoglycan Antibody [NBP1-90306] - Staining of human heart muscle shows distinct positivity of intercalated discs in myocytes.
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Bioinformatics

Gene Symbol SEMA4D
Entrez