Secretory phospholipase A2 Type V Antibody

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Immunohistochemistry-Paraffin: Secretory phospholipase A2 Type V Antibody [NBP1-39690] - Staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen ...read more

Order Details


    • Catalog Number
      NBP1-39690
    • Availability
      Product Discontinued

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Secretory phospholipase A2 Type V Antibody Summary

Immunogen
murine group V sPLA2 amino acids 79-94 (CAIRTQSYDYRYTNGL)
Clonality
Polyclonal
Host
Rabbit
Gene
PLA2G5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
For IHC-P: Use heat induced antigen retrieval in pH 6.0 citrate buffer.

Reactivity Notes

Mouse, Human, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH 7.4), 0.5 mg/ml BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Secretory phospholipase A2 Type V Antibody

  • Ca2+-dependent phospholipase A2
  • calcium-dependent phospholipase A2
  • DKFZp686C2294
  • EC 3.1.1.4
  • Group V phospholipase A2
  • GV-PLA2
  • hVPLA(2)
  • MGC46205
  • Phosphatidylcholine 2-acylhydrolase 5
  • phospholipase A2, group V
  • PLA2-10

Background

Secretory phospholipase A2 Type V (PLA2) catalyzes the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids and is one of the isoforms in the growing list of the PLA2 enzyme family. This enzyme, rather than sPLA2 (Type II), is responsible for arachidonic acid mobilization leading to prostaglandin production in macrophages and mast cells. Consistent with this role, sPLA2 (Type V) is associated with the golgi apparatus, nuclear envelope, and plasma membrane in murine bone marrow-derived mast cells. sPLA2 (Type V) has been cloned from a variety of species including human, mouse and rat. The enzyme is expressed in heart, lung, placenta and spleen, as well as P388D1 macrophages and mast cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PLA2G5
Entrez
Uniprot